English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/142694
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorReppert, Emily-
dc.contributor.authorGalindo, Ruth C.-
dc.contributor.authorKocan, Katherine M.-
dc.contributor.authorBlouin, Edmour F.-
dc.contributor.authorFuente, José de la-
dc.date.accessioned2017-01-18T12:30:55Z-
dc.date.available2017-01-18T12:30:55Z-
dc.date.issued2013-
dc.identifierdoi: 10.1111/tbed.12120-
dc.identifierissn: 1865-1674-
dc.identifiere-issn: 1865-1682-
dc.identifier.citationTransboundary and Emerging Diseases 60(s2): 93-96 (2013)-
dc.identifier.urihttp://hdl.handle.net/10261/142694-
dc.description.abstractAnaplasma phagocytophilum, first identified as a pathogen of sheep in Europe, more recently has been recognized as an emerging tick-borne pathogen of humans in the U.S. and Europe. Transmission of A. phagocytophilum is reported to be by ticks, primarily of the genus Ixodes. While mechanical and transplacental transmission of the type genus organism, A. marginale, occur in addition to tick transmission, these modes of transmission have not been considered for A. phagocytophilum. Recently, we developed a sheep model for studying host-tick-pathogen interactions of the human NY-18 A. phagocytophilum isolate. Sheep were susceptible to infection with this human isolate and served as a source of infection for I. scapularis ticks, but they did not display clinical signs of disease, and the pathogen was not apparent in stained blood smears. In the course of these experiments, one sheep unexpectedly gave birth to a lamb 5 weeks after being experimentally infected by inoculation with the pathogen propagated in HL-60 cells. The lamb was depressed and not feeding and was subsequently euthanized 18 h after birth. Tissues were collected at necropsy for microscopic examination and PCR to confirm A. phagocytophilum infection. At necropsy, the stomach contained colostrum, the spleen was moderately enlarged and thickened with conspicuous lymphoid follicles, and mesenteric lymph nodes were mildly enlarged and contained moderate infiltrates of eosinophils and neutrophils. Blood, spleen, heart, skin and cervical and mesenteric lymph nodes tested positive for A. phagocytophilum by PCR, and sequence analysis confirmed that the lamb was infected with the NY-18 isolate. Transplacental transmission should therefore be considered as a means of A. phagocytophilum transmission and may likely contribute to the epidemiology of tick-borne fever in sheep and other mammals, including humans.-
dc.description.sponsorshipThis research was supported by the Walter R. Sitlington Endowed Chair for Food Animal Research to KMK, Center for Veterinary Health Sciences, Research Advisory Committee grants to KMK, RCG and the Spanish Ministerio de Ciencia e Innovación (MICINN) project BFU2008-01244/BMC to JF.-
dc.publisherWiley-Blackwell-
dc.rightsclosedAccess-
dc.subjectSheep-
dc.subjectHuman isolate-
dc.subjectTick-borne-
dc.subjectTransplacental transmission-
dc.subjectAnaplasma phagocytophilum-
dc.titleDemonstration of transplacental transmission of a human isolate of Anaplasma phagocytophilum in an experimentally infected sheep-
dc.typeartículo-
dc.identifier.doi10.1111/tbed.12120-
dc.date.updated2017-01-18T12:30:55Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderSitlington Endowment Funds-
dc.contributor.funderOklahoma State University-
dc.contributor.funderResearch Grants Council (Hong Kong)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/100007069es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
Appears in Collections:(IREC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.