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Título

Subolesin: A candidate vaccine antigen for the control of cattle tick infestations in Indian situation

AutorShakya, Mukesh; Fuente, José de la ; Ghosh, Srikanta
Palabras claveSubolesin
Rhipicephalus (Boophilus) microplus
Anti-tick vaccine
Fecha de publicación2014
EditorElsevier
CitaciónVaccine 32(28): 3488-3494 (2014)
ResumenIdentification of cross-protective tick vaccine antigens is a challenging area of veterinary research. To address this challenge, a recently identified candidate tick protective antigen, Subolesin (SUB), was targeted in this research. The conservation of subolesin ortholog of Hyalomma anatolicum and Rhipicephalus (Boophilus) microplus across different Indian strains was 98.1-99.4% (within species), while at the amino acid level SUB sequence homology was ≥53.2% (between tick species). Recombinant R. (B.) microplus SUB (rBmSu) was produced in Escherichia coli and characterized. Cross-bred cattle male calves (N= 10) were immunized with three doses of 100. μg each of the rBmSu emulsified in 10% Montanide 888 at monthly intervals on days 0, 30 and 60. The control group was injected with PBS in 10% Montanide 888. For the first tick challenge, calves were infested with larvae of R. (B.) microplus generated from 100. mg eggs 2 weeks after last immunization (day 75). The immunization resulted in 16.3%, 8.0%, 9.4%, and 26.1% reduction in female tick numbers (DT), weight (DW), oviposition (DO) and egg fertility (DF), respectively, when compared to controls. In the subsequent challenge on day 105, DT, DW, DO and DF were reduced by 9.0%, 4.1%, 8.6%, and 24.2%, respectively, when compared to controls. The vaccine efficacy (E) was equal to 44.0% and 37.2% after the first and second challenges, respectively. The results showed a positive correlation between antibody titers for both total IgG and IgG1 and E in the second but not in the first tick challenge. These results suggested the possibility of developing a SUB-based vaccine for control of cattle tick infestations under Indian conditions.
URIhttp://hdl.handle.net/10261/142545
DOI10.1016/j.vaccine.2014.04.053
Identificadoresdoi: 10.1016/j.vaccine.2014.04.053
issn: 0264-410X
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