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Título

Un ecosistema de innovación abierto al servicio del aprendizaje y la investigación

AutorVeramendi, Jon
Fecha de publicaciónjul-2015
CitaciónIX Congreso de la Federación Española de Biotecnólogos (2015)
ResumenPlastid transformation offers severa! advantages over nuclear transformation, being the main one the accumulation of exceptionally large quantities of foreign proteins, up lo 70% of the total soluble protein. Additional advantages of this recombinant protein expression system include foreign DNA integration via homologous recombination, transgene containment via maternal inheritance of plastids, lack of gene silencing and multigene expression in a single transformation step via operons beca use of the ability of chloroplasts to process polycistrons. During the talk, results on the production of different foreign proteins with different applications will be presentad. The biopharmaceutical human serum albumin was expressed at high levels, inducing the formation of inclusion bodies within the chloroplast. The development of a peptide vaccine against the canine parvovirus showed immunogenicity in model animals (mouse and rabbit) developing neutralizing antibodies. A human vaccine against papillomavirus was produced in toba eco chloroplasts by expression of the majar structural L 1 protein of the capsid. This protein was able to assemble into virus like particles in the stroma of the chloroplast that were highly immunogenic in mice. Severa! chloroplast-derived antigens against toxoplasmosis were used as oral vaccines, and resultad in a significant decrease in the brain cyst load of mice comparad to control animals. In addition to biopharmaceuticals, chloroplast transformation can be used to improve agronomical traits. For example, we were able to enhance starch accumulation in tobacco leaves by the overexpression of thioredoxin f. Commercial tobacco cultivars grown under field conditions were used as a feedstock for ethanol production. After leaf and stalk processing, up to 40 % more ethanol was obtained from transplastomic plants than their wild-type controls.
DescripciónTrabajo presentado en el IX Congreso de la Federación Española de Biotecnólogos (IX Congress of the Spanish Federation of Biotechnologists - BAC 2015), celebrado en Salamanca del 8 al 10 de julio de 2015.
URIhttp://hdl.handle.net/10261/142389
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