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Effect of Spodoptera exigua iflavirus co-inoculation on the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)

AuthorsCarballo, Arkaitz ; Murillo, Rosa ; Williams, Trevor G.; Caballero, Primitivo
Issue Date2016
PublisherInternational Organisation for Biological and Integrated Control
CitationIOBC-WPRS Bulletin 113: 127-130 (2016)
AbstractBaculovirus are natural pathogens that can produce epizootics in agricultural ecosystems. The Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control this pest in sweet pepper crop systems of southern Spain. Studies on SeMNPV persistence in covertly infected adults revealed the presence of RNA viruses in insect colonies. These were identified as two iflaviruses (SeIV1 and SeIV2). Subsequent experiments indicated that SeMNPV and SeIV could be detected in both field and laboratory insect populations using PCR-based techniques. In this study, we evaluated the effect of SeIV1 co-infection on the insecticidal characteristics of SeMNPV. For this, feeding-drop bioassays were performed on second instar larvae by inoculating at the same time one of five SeMNPV inoculum concentrations and one of the following iflavirus treatments: i) SeIV1; ii) SeIV2; iii) SeIV1+SeIV2; and mock-infected control. Overall, iflavirus co-inoculation consistently reduced median lethal concentrations (LC50) for SeMNPV compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. Adults survivors of a sublethal SeMNPV treatment were examined for covert infection and SeMNPV DNA were found to be present at a high prevalence in all treatments. In conclusion, simultaneous SeMNPV-SeIV co-infection resulted in improved pathogenicity of SeMNPV and did not alter the capacity of SeMNPV for vertical transmission.
DescriptionTrabajo presentado en la 15th Meeting of the IOBC-WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”, celebrada en Riga del 7 al 11 de junio de 2015.
Identifiersisbn: 978-92-9067-296-8
Appears in Collections:(IDAB) Libros y partes de libros
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