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Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats

AutorAlmog, Mara; Korngreen, Alon
Palabras claveVoltage-Gated Ca2+
Pyramidal Neurons
Fecha de publicación1-abr-2009
EditorPublic Library of Science
CitaciónPLoS ONE. 2009; 4(4): e4841
ResumenNeuronal voltage-gated Ca2+ channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca2+ channels is generating regenerative dendritic Ca2+ spikes. However, the Ca2+ dependent mechanisms used to create these spikes are only partially understood. To start investigating this mechanism, we set out to kinetically and pharmacologically identify the sub-types of somatic voltage-gated Ca2+ channels in pyramidal neurons from layer 5 of rat somatosensory cortex, using the nucleated configuration of the patch-clamp technique. The activation kinetics of the total Ba2+ current revealed conductance activation only at medium and high voltages suggesting that T-type calcium channels were not present in the patches. Steady-state inactivation protocols in combination with pharmacology revealed the expression of R-type channels. Furthermore, pharmacological experiments identified 5 voltage-gated Ca2+ channel sub-types – L-, N-, R- and P/Q-type. Finally, the activation of the Ca2+ conductances was examined using physiologically derived voltage-clamp protocols including a calcium spike protocol and a mock back-propagating action potential (mBPAP) protocol. These experiments enable us to suggest the possible contribution of the five Ca2+ channel sub-types to Ca2+ current flow during activation under physiological conditions.
Descripción11 pages, 7 figures.-- PMID: 19337371 [PubMed].-- PMCID: PMC2659773.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0004841
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