English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/14207
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 8 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Characterization of voltage-gated Ca(2+) conductances in layer 5 neocortical pyramidal neurons from rats

AutorAlmog, Mara; Korngreen, Alon
Palabras claveVoltage-Gated Ca2+
Pyramidal Neurons
Rats
Neocortex
Fecha de publicación1-abr-2009
EditorPublic Library of Science
CitaciónPLoS ONE. 2009; 4(4): e4841
ResumenNeuronal voltage-gated Ca2+ channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca2+ channels is generating regenerative dendritic Ca2+ spikes. However, the Ca2+ dependent mechanisms used to create these spikes are only partially understood. To start investigating this mechanism, we set out to kinetically and pharmacologically identify the sub-types of somatic voltage-gated Ca2+ channels in pyramidal neurons from layer 5 of rat somatosensory cortex, using the nucleated configuration of the patch-clamp technique. The activation kinetics of the total Ba2+ current revealed conductance activation only at medium and high voltages suggesting that T-type calcium channels were not present in the patches. Steady-state inactivation protocols in combination with pharmacology revealed the expression of R-type channels. Furthermore, pharmacological experiments identified 5 voltage-gated Ca2+ channel sub-types – L-, N-, R- and P/Q-type. Finally, the activation of the Ca2+ conductances was examined using physiologically derived voltage-clamp protocols including a calcium spike protocol and a mock back-propagating action potential (mBPAP) protocol. These experiments enable us to suggest the possible contribution of the five Ca2+ channel sub-types to Ca2+ current flow during activation under physiological conditions.
Descripción11 pages, 7 figures.-- PMID: 19337371 [PubMed].-- PMCID: PMC2659773.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0004841
URIhttp://hdl.handle.net/10261/14207
DOI10.1371/journal.pone.0004841
ISSN1932-6203
Aparece en las colecciones: (IC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
pone.0004841.pdf598,15 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.