English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/141997
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Biophysical screening for identifying pharmacological chaperones and inhibitors against conformational and infectious diseases

AuthorsVelazquez-Campoy, Adrian; Sancho, Javier ; Abian, Olga ; Vega, Sonia
Issue Date2016
CitationCurrent drug targets 17: 1492- 1505 (2016)
AbstractExperimental and computational screenings are currently widespread tools for identifying either potential ligands for a given target or potential targets for a given chemical compound. In particular, ligand-induced stabilization against thermal denaturation (or thermal shift assay) is an easy and convenient experimental procedure for finding compounds able to control the activity of a protein target (e.g., allosteric or competitive inhibitors interfering with its activity, allosteric activators enhancing its activity, or pharmacological chaperones avoiding aggregation, unfolding or degradation). Despite its simplicity the thermal shift assay does not lack some important drawbacks. Here we describe this methodology, its application to structured and unstructured protein targets, and we discuss successful cases of identification of bioactive compounds for conformational disorders associated to loss of function (phenylketonuria) and infectious diseases (gastric ulcer and hepatitis C).
URIhttp://hdl.handle.net/10261/141997
DOI10.2174/1389450117666160201110449
Identifiersdoi: 10.2174/1389450117666160201110449
issn: 1873-5592
Appears in Collections:(IQFR) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.