English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/141994
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Functional genomics of tick vectors challenged with the cattle parasite Babesia bigemina

AuthorsDomingos, Ana; Antunes, Sandra; Villar, Margarita ; Fuente, José de la
KeywordsSubtractive hybridization
Boophilus
Babesia
RNA interference
Vaccines
Rhipicephalus
Tick
Genomics
Issue Date2015
PublisherSpringer
Humana Press
CitationVeterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies: 475-489 (2015)
SeriesMethods in Molecular Biology 1247
AbstractTicks are obligate hematophagous ectoparasites considered as vectors of animal diseases, having a huge economic impact in cattle industry. Babesia spp. are tick-borne pathogens that cause a disease called babesiosis in a wide range of animals and in humans. Control of tick infestations is mainly based on the use of acaricides, which have limited efficacy reducing tick infestations, mostly due to wrong usage, and is often accompanied by the selection of acaricide-resistant ticks, environmental contamination, and contamination of milk and meat products. Vaccines affecting both vector and pathogens constitute new control strategies for tick and tick-borne diseases and are, therefore, a good alternative to chemical control. In this chapter we describe the identification of Rhipicephalus (Boophilus) annulatus genes differentially expressed in response to infection with B. bigemina by using suppression-subtractive hybridization (SSH), which allows the identification of differentially expressed genes. The results of the SSH studies are validated by real-time reverse transcription (RT)-PCR. Functional analyses are conducted by RNAi on selected R. annulatus genes to determine their putative role in B. bigemina–tick interactions. Gathered data may be useful for the future development of improved vaccines and vaccination strategies to control babesiosis
URIhttp://hdl.handle.net/10261/141994
DOI10.1007/978-1-4939-2004-4_32
Identifiersdoi: 10.1007/978-1-4939-2004-4_32
isbn: 978-1-4939-2003-7
Appears in Collections:(IREC) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.