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Biodiversity assesment of Mediterranean Sepiolidae by DNA barcoding

AutorFernández-Álvarez, Fernando Ángel ; Sánchez, Pilar ; Martins, Catarina; Cuesta-Torralvo, Elisabeth; Villanueva, Roger
Fecha de publicaciónnov-2015
EditorCephalopod International Advisory Council
CitaciónCephalopod International Advisory Council Conference (2015)
ResumenDNA barcoding, a method for molecular identification at species-level, has been developed and widely used in many biological disciplines. It is especially useful for taxonomically-difficult groups which need of highly-specialized methods for identification, or those that can only be specifically assigned in some phases of their vital cycle. However, these methods are constrained by the taxonomic reliability of the previous existing data. The family Sepiolidae Leach, 1817 is formed by 15 genera and more than 60 species around the world. Seven of these genera are represented in the Mediterranean Sea, where 16 bobtail squid species are known. Members of the two more diverse genera, Sepiola Leach, 1817 (with 8 species) and Sepietta Naef, 1912, could only be identified when they are fully-mature. Previous molecular works have been performed in Mediterranean sepiolids. Nevertheless, inconsistencies in the specific identity between supposedly conspecifics are yet to be resolved. Here, a fragment cytochrome oxidase I of 12 species covering all the Mediterranean sepiolid genera was obtained and their bodies were vouchered to ensure the correct identification of the specimen. We provide a correct barcode for each of these species, resolve some of the previous problematic sequences and evaluate the use of DNA barcoding in this group of organisms
DescripciónCephalopod International Advisory Council (CIAC) Conference, 10-14 November 2015, Hakodate, Japan
URIhttp://hdl.handle.net/10261/141925
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