English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/141769
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen

AuthorsContreras, Marinela; Moreno-Cid, Juan A. ; Domingos, Ana; Canales, Mario ; Díez-Delgado, Iratxe ; Pérez de Lastra, José Manuel ; Sánchez, Emilio; Merino, Octavio ; Ayllón, Nieves ; Boadella, Mariana ; Villar, Margarita ; Gortázar, Christian ; Fuente, José de la
KeywordsTick
Subolesin
MSP1a
Immunology
Endotoxin
Chimera
Vaccine
Issue Date2015
PublisherElsevier
CitationTicks and Tick-borne Diseases 6(6): 820-828 (2015)
AbstractTicks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-. Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.
URIhttp://hdl.handle.net/10261/141769
DOI10.1016/j.ttbdis.2015.07.010
Identifiersdoi: 10.1016/j.ttbdis.2015.07.010
e-issn: 1877-9603
issn: 1877-959X
Appears in Collections:(IREC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.