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Título

Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen

AutorContreras, Marinela; Moreno-Cid, Juan A. ; Domingos, Ana; Canales, Mario ; Díez-Delgado, Iratxe ; Pérez de Lastra, José Manuel ; Sánchez, Emilio; Merino, Octavio ; Ayllón, Nieves ; Boadella, Mariana ; Villar, Margarita ; Gortázar, Christian ; Fuente, José de la
Palabras claveTick
Subolesin
MSP1a
Immunology
Endotoxin
Chimera
Vaccine
Fecha de publicación2015
EditorElsevier
CitaciónTicks and Tick-borne Diseases 6(6): 820-828 (2015)
ResumenTicks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-. Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.
URIhttp://hdl.handle.net/10261/141769
DOI10.1016/j.ttbdis.2015.07.010
Identificadoresdoi: 10.1016/j.ttbdis.2015.07.010
e-issn: 1877-9603
issn: 1877-959X
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