English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/141767
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Comparative effects of "defective" genotypes in NPV populations: their roles in SfMNPV vs SeMNPV

AutorSerrano, Oscar ; Simón, Oihane ; Muñoz, Delia ; Williams, Trevor G.; Caballero, Primitivo
Palabras claveDefective genotyes
SfMNPV
SeMNPV
Transmissibility
Fecha de publicaciónjul-2010
Citación43rd Annual Meeting of the Society for Invertebrate Pathology (2010)
10th International Colloquium on Invertebrate Pathology (2010)
ResumenThe Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida, US (SeUS2) is a complex mixture of genotypes. The genotypic structure of this isolate is very similar to that of the S. frugiperda MNPV isolated from Nicaragua (SfNIC), which is composed of at least nine genotypes. Both isolates present defective genotypes with similar deletions that lack peroral infectivity. The SfNIC defective genotype (SfNIC-C) plays a functional role in the transmissibility of the viral population since its presence increased the pathogenicity of OBs in co-occluded mixtures of complete and defective genotypes. The aim of this study was to determine the functional role of the defective genotype SeUS2-C, that is not infective per os, in the SeUS2 isolate. For this, different experimental populations were constructed that included the complete genotype SeUS2-A and the deletion mutant genotype SeUS2-C at different proportions in OB mixtures or co-occluded genotypic mixtures. The SeUS2-C was the most abundant isolated genotype in cell culture accounting for ~75% of plaques. However, the proportion of SeUS2-C in the viral population as determined by semiquantitative PCR was ~25%. SeUS2-A OBs were as pathogenic as wild-type OBs. As predicted, the potency of the mixtures of SeUS2-A OBs and SeUS2-C OBs was dependent on the proportion of SeUS2-A present in the mixture. In contrast, the co-occluded mixtures that included the defective genotype SeUS2-C at the proportions of 10 and 25%, respectively, were as pathogenic asSeUS2-A. However, the presence of a higher proportion of SeUS2-C in the mixtures reduced the biological activity of OBs. We conclude that the defective genotype SeUS2-C appears to persist as a parasitic genotype in the SeUS2 population. This contrasts with the role of defective genotypes in the SfMNPV population that potentiate OB potency.
DescripciónTrabajo presentado en la 43rd Annual Meeting of the Society for Invertebrate Pathology y 10th International Colloquium on Invertebrate Pathology, celebrado en Trabzon (Turquía) del 11 al 15 de julio de 2010. The Final Meeting of COST862 Action: Bacterial Toxins for Insect Control.
URIhttp://hdl.handle.net/10261/141767
Aparece en las colecciones: (IDAB) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.