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Título

Selection of red deer spermatozoa with different cryoresistance using density gradients

AutorGarcía-Álvarez, Olga; Soler, Ana J. ; Maroto-Morales, Alejandro ; Maulen, Z.; Iniesta-Cuerda, María; Martín-Maestro, A.; Fernández-Santos, M. Rocío ; Garde, José Julián
Fecha de publicación2016
EditorWiley-Blackwell
CitaciónReproduction in Domestic Animals 51(6): 895-900 (2016)
ResumenThe objective of sperm selection media is selecting the best spermatozoa and to remove seminal plasma and diluent for using them in assisted reproductive techniques. It is known that individuals show different cryoresistance in response to the same freezing procedure. Our hypothesis was that the efficacy of selection media could be dissimilar for samples with different sperm quality after thawing. Epididymal sperm samples from mature Iberian red deer were collected and frozen. Males were classified as with high post-thaw sperm quality when sperm motility (SM) ≥ 70%, or as with low post-thaw sperm quality when SM ≤ 69%. Samples were centrifuged using the following density gradients (DG): Percoll, Puresperm and Bovipure, and several functional sperm parameters were assessed after sperm selecting and washing. Males classified with high sperm quality had higher post-thawing values (p >.05) for all parameters evaluated, except for linearity index, than those categorized as low sperm quality. After selection, some sperm characteristics improved (viability, apoptosis and mitochondrial activity) for both groups, showing the males with high sperm quality higher values in all sperm parameters except for kinematic traits and DNA fragmentation index (%DFI), regardless of DG. Bovipure yield lower values of sperm motility, viability, apoptosis and mitochondrial activity in relation to Percoll and Puresperm considering both quality groups. There was an interaction between the type of DG and sperm quality group for sperm viability (p =.040) and apoptosis (p =.003). Thus, Percoll selected less live and more apoptotic spermatozoa than Puresperm and Bovipure for males with low sperm quality. In conclusion, the DG are more efficient selecting spermatozoa from samples with high sperm quality, acting differently depending on initial sperm quality.
URIhttp://hdl.handle.net/10261/141689
DOI10.1111/rda.12755
Identificadoresdoi: 10.1111/rda.12755
e-issn: 1439-0531
issn: 0936-6768
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