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Thermal tolerance and immune response of the sea cucumber Parastichopus regalis exposed to a gradient of high temperatures

AutorGalimany, Eve ; Baeta, Marc ; Ramón, Montserrat
Fecha de publicación25-may-2015
Citación15th International Echinoderm Conference (2015)
ResumenSea cucumbers are echinoderms from the class Holothuroidea. They have a worm-like body with a central elongated cavity filled with coelomic fluid. Within the fluid there are cells and molecules that participate in the cellular and humoral immune response. The free cells involved in the immune response are called coelomocytes and they have very different functions, i.e. phagocytosis, encapsulation, superoxide anion production, superoxide dismutase activity and total nitric oxide synthase. There are very few studies focused on sea cucumbers immunology and their response to environmental stressors. The sea cucumber Parastichopus regalis (Cuvier, 1817) is a holothurian species of the family Stichopodidae inhabiting sandy bottoms from the northwestern Mediterranean, eastern Atlantic from the south of the Canary Islands to the north of Ireland, and western Atlantic, Antilles and Gulf of Mexico. These species is commonly found below 100m depth in the Mediterranean, where the water temperature is maintained around 13ºC. This species is commercialized for human consumption in Catalonia (NW Mediterranean), where it is currently the most expensive sea product on the market. P. regalis could be a potential species for an integrated multitrophic culture but aquaculture facilities are located in shallow waters, where water temperature reaches values up to 28ºC, much higher than their typical temperature habitat. Therefore, we exposed P. regalis to a gradient of high temperatures to determine its thermal tolerance and investigated their immune response. Individuals of Parastichopus regalis were exposed to a temperature gradient (13ºC, 18ºC, 23ºC and 28ºC) for two weeks and the survival and immune response (types, density and percentage of phagocytic and apoptotic coelomocytes) was studied at day 0, 2, 7 and 14, and after a recovery period of 1 week at 13ºC. Results showed that 90% of the sea cucumbers exposed to 28ºC died before 48h and they had 50% survivorship after 2 weeks of exposure to 23ºC. The proportion of coelomocyte types did not change along the study for animals exposed to 23º or 18ºC. Coelomocytes density increased in time in the 23ºC for both treatments (high temperature and control). P. regalis exposed to 18ºC and 23ºC had significantly less phagocytic and apoptotic coelomocytes during the two weeks of exposure that control ones. All the differences found were not observed after the recovery period. Results show that P. regalis seem to have a temperature maximum tolerance of 18ºC above which their survivorship is compromised, although immunodepression is already apparent at that temperature
Descripción15th International Echinoderm Conference, 25-29 may 2015, Playa del Carmen
URIhttp://hdl.handle.net/10261/141501
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