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Title

The presence of small ruminant lentiviruses in Mexican Pelibuey sheep

AuthorsSánchez, José H.; Martínez, Humberto A.; García, María M.; García, María M.; Garrido, Germán; Gómez, Luis; Aguilar, José A.; Andrés, Damián F. de ; Reina, Ramsés ; Ramírez, Hugo
KeywordsSmall ruminant lentiviruses
Synthetic peptides
Pelibuey breed
ELISA
PCR
Issue DateNov-2016
PublisherElsevier
CitationTheriogenology 86(8): 1953-1957.e1 (2016)
AbstractThe transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta.
Publisher version (URL)http://doi.org/10.1016/j.theriogenology.2016.06.017
URIhttp://hdl.handle.net/10261/141421
DOI10.1016/j.theriogenology.2016.06.017
Identifiersissn: 0093-691X
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