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Leghemoglobins with Nitrated Hemes in Legume Root Nodules

AuthorsBecana Ausejo, Manuel ; Navascués Ortega, Joaquín ; Pérez-Rontomé, Carmen ; Walker, F. A.; Desbois, A.; Abian, J.
Keywordsfree radicals
nitrogen fixation
nitrated heme
nodule senescence
reactive nitrogen species
Issue DateJul-2015
PublisherJohn Wiley & Sons
CitationBecana M, Navascués J, Pérez-Rontomé C, Walker FA, Desbois A, Abian J. Leghemoglobins with Nitrated Hemes in Legume Root Nodules. In: Biological Nitrogen Fixation (ed F. J. de Bruijn), John Wiley & Sons, Inc, Hoboken, NJ, USA. Chapter 70: 705-713. doi: 10.1002/9781119053095.ch70 (2015)
AbstractGlobins are widespread in all kingdoms of life, where they fulfil multiple functions such as efficient O2 transport and modulation of nitric oxide bioactivity. Plants contain nonsymbiotic (classes 1 and 2) and truncated (class 3) hemoglobins in virtually all tissues. In legume nodules, however, the most abundant hemoglobins are the symbiotic leghemoglobins (Lbs) that facilitate O2 diffusion to the N2-fixing bacteroids. Virtually nothing is known about the green derivatives of Lbs generated during nodule senescence. Here, we characterize green modified forms of Lbs, termed Lbam, Lbcm,, and Lbdm, of soybean nodules. They have identical globins to the parent red Lbs, but their hemes are nitrated. By combining diverse spectroscopies with reconstitution of the apoprotein with protoheme or mesoheme, we show that the nitro (NO2) group is on the 4-vinyl. In vitro nitration of Lba with excess nitrite produced several isomers of nitrated heme, one of which is identical to those found in vivo. We show that nitration requires binding of nitrite to the heme iron, and that the reactive nitrogen species involved derives from nitrous acid (HNO2) and is most probably the nitronium cation (NO2+). The identification of these green Lbs provides conclusive evidence that highly oxidizing and nitrating species are increasingly produced in senescent nodules, leading to nitrosative stress.
Description36 Pags.- 5 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/
Publisher version (URL)http://dx.doi.org/10.1002/9781119053095.ch70
ISBN9781118637043 (Print)
9781119053095 (Online)
Appears in Collections:(EEAD) Libros y partes de libros
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