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Deciphering the role of aquaporins during sperm motility in the marine teleost gilthead seabream

AuthorsBoj, Mónica; Chauvigné, François ; Cerdà, Joan
Issue Date12-Jun-2015
PublisherSocietat Catalana de Biologia
CitationAvenços en Recerca en Aqüicultura. Programa: 2-3 (2015)
AbstractIIn the marine teleost gilthead seabream (Sparus aurata) different aquaporin water channels, such as Aqp1aa, -1ab, -7 and -8b, are expressed during the hyperosmotic induction of spermatozoon motility in seawater (SW). However, the physiological roles of these channels are unknown. Single and double immunofluorescence microscopy studies indicate that Aqp1aa and -7 are respectively localized in the entire flagellum or the head, while Aqp1ab and -8b are both in the head and the anterior tail of ejaculated spermatozoa. Upon SW activation, Aqp1ab and -8b are rapidly phosphorylated and translocated to the head plasma membrane and the mitochondrion, respectively, whereas Aqp1aa and -7 remain unchanged. Immunological inhibition of Aqp1aa function reduced the rise of intracellular Ca2+ that normally occurs upon activation, and strongly inhibited sperm motility. Impaired Aqp1aa function also prevented the intracellular trafficking of Aqp8b to the mitochondrion, where it acts as a peroxiporin allowing H2O2 efflux and ATP production during flagellar motility. However, restoring the Ca2+ levels in spermatozoa with immunosuppressed Aqp1aa fully rescued mitochondrial Aqp8b accumulation and sperm motility. In contrast, exposure of sperm to Aqp1ab and -7 antibodies did not affect motility during the initial phase of activation, but latently compromised the trajectory and the pattern of movement. These data reveal the coordinated action of spatially segregated aquaporins during sperm motility activation in seabream, where flagellar-localized Aqp1aa plays a dual Ca2+ -dependent role controlling the initiation of motility and the activation of mitochondrial Aqp8b-mediated detoxification mechanisms, while Aqp1ab and -7 in the head and anterior tail direct the motion pattern. These findings provide new insights into the molecular mechanisms regulating sperm motility in marine teleosts and the causes of male infertility under culture conditions
DescriptionJornada Anual de la Secció d’Aqüicultura de la Societat Catalana de Biologia, Avenços en Recerca en Aqüicultura, 12 de Juny de 2015, Barcelona.-- 2 pages
Publisher version (URL)http://scb.iec.cat/jornada-de-recerca-en-aquicultura/
Appears in Collections:(ICM) Comunicaciones congresos
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