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Environmental effects during early development mediated by epigenetic mechanisms in fish

AuthorsPiferrer, Francesc ; Anastasiadi, Dafni ; Díaz, Noelia ; Sucarrats, Ivet
Issue Date10-Mar-2015
PublisherSocietat Catalana de Biologia
CitationV Jornada de Cromatina i Epigenètica. Program: 6-7 (2015)
AbstractIn contrast to mammals, fertilization and embryonic development are external in fish. Thus, fish are ideal models for studying how environmental information received during early development is integrated through epigenetic mechanisms. Epigenetic modifications can affect gene expression permanently and this may influence the potential of an organism to respond to these environmental cues later in life, with consequences not only for the sustainability of natural populations in a scenario of global change but also for optimizing animal production. Recently, we showed that in oneyearold European sea bass the DNA methylation levels in the promoter of gonadal aromatase were higher when the fish were exposed to elevated temperature from 0 to 60 days post fertilization (dpf) compared to fish raised at natural temperature (NavarroMartín et al. 2011. PLoS Genetics 7(12): e1002447). In order to narrow down the developmental period which is crucial for the establishment of DNA methylation marks by temperature, we studied global DNA methylation in larvae having experienced high temperature exposure at different time points and for variable duration up to 60 dpf. Moving on to another perspective, we have also studied the effects of temperature treatment during larval development, as well as the ones of farming conditions in the muscle and testis of 3yearold fish. We detected differentially methylated cytosines (DMC) in both tissues and both experiments, most of which neighboring genes and their regulatory elements, while attempting to correlate these data with their corresponding transcriptomic profile, with regard to establishing a functional relationship. Furthermore, we conducted experiments to explore the potential implication of histone modifications in the regulation of expression of key genes. In addition, we are currently examining the DNA methylation levels of a panel of 40 functionally important genes in gonads and muscle of immature, reproductively active and senescent fish. Our experiments together highlight the significance of the early environment an organism experiences for the establishment of epigenetic marks with long lasting phenotypic consequences
DescriptionV Jornada de Cromatina i Epigenètica organitzada per la Secció de Biologia Molecular de la Societat Catalana de Biologia (SCB) amb el Barcelona Chromatin Club (BCC) : V Annual Chromatin and Epigenetics Symposium organized by the Molecular Biology section of the Catalan Society of Biology (SCB) and the Barcelona Chromatin Club (BCC), 10 de març de 2015, Barcelona.-- 2 pages
Publisher version (URL)http://scb.iec.cat/v-jornada-de-cromatina-i-epigenetica/
Appears in Collections:(ICM) Comunicaciones congresos
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