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dc.contributor.authorFukudome, Mitsutakaes_ES
dc.contributor.authorCalvo-Beguería, Lauraes_ES
dc.contributor.authorKado, Tomohiroes_ES
dc.contributor.authorOsuki, Ken-ichies_ES
dc.contributor.authorRubio Luna, María Carmenes_ES
dc.contributor.authorMurakami, Ei-ichies_ES
dc.contributor.authorNagata, Makies_ES
dc.contributor.authorKucho, Ken-ichies_ES
dc.contributor.authorSandal, Nielses_ES
dc.contributor.authorStougaard, Jenses_ES
dc.contributor.authorBecana Ausejo, Manueles_ES
dc.contributor.authorUchiumi, Toshikies_ES
dc.identifier.citationFukudome M, Calvo-Beguería L, Kado T, Osuki K, Rubio MC, Murakami E, Nagata M, Kucho K, Sandal N, Stougaard J, Becana M, Uchiumi T. Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus–Mesorhizobium loti symbiosis. Journal of Experimental Botany 67 (17): 5275-5283 (2016)es_ES
dc.description36 Pags.- 10 Figs.- 5 Tabls. The definitive version is available at: http://jxb.oxfordjournals.org/es_ES
dc.description.abstractLeghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia–legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5′-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo. The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels.es_ES
dc.description.sponsorshipThis project was supported by Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects (Japan), by Danish National Research Foundation grant DNRF79 (Denmark), and by Ministry of Economy and Competitiveness grant AGL2014-53717-R (Spain).es_ES
dc.publisherOxford University Presses_ES
dc.subjectLotus japonicuses_ES
dc.subjectMesorhizobium loties_ES
dc.subjectnitric oxidees_ES
dc.subjectnitrogen fixationes_ES
dc.subjectnodulation mutantses_ES
dc.titleHemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus–Mesorhizobium loti symbiosises_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderJapan Society for the Promotion of Sciencees_ES
dc.contributor.funderDanish National Research Foundationes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
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