English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/141178
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus–Mesorhizobium loti symbiosis

AuthorsFukudome, Mitsutaka; Calvo-Beguería, Laura ; Kado, Tomohiro; Osuki, Ken-ichi; Rubio Luna, María Carmen ; Murakami, Ei-ichi; Nagata, Maki; Kucho, Ken-ichi; Sandal, Niels; Stougaard, Jens; Becana Ausejo, Manuel ; Uchiumi, Toshiki
KeywordsHemoglobin
Lotus japonicus
Mesorhizobium loti
nitric oxide
nitrogen fixation
nodulation mutants
symbiosis
Issue DateJul-2016
PublisherOxford University Press
CitationFukudome M, Calvo-Beguería L, Kado T, Osuki K, Rubio MC, Murakami E, Nagata M, Kucho K, Sandal N, Stougaard J, Becana M, Uchiumi T. Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus–Mesorhizobium loti symbiosis. Journal of Experimental Botany 67 (17): 5275-5283 (2016)
AbstractLeghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia–legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5′-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo. The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels.
Description36 Pags.- 10 Figs.- 5 Tabls. The definitive version is available at: http://jxb.oxfordjournals.org/
Publisher version (URL)http://dx.doi.org/10.1093/jxb/erw290
URIhttp://hdl.handle.net/10261/141178
DOI10.1093/jxb/erw290
ISSN0022-0957
E-ISSN1460-2431
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
BecanaM_JExpBot_2016.pdf2,03 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.