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dc.contributor.authorMaiques, Elisaes_ES
dc.contributor.authorQuiles-Puchalt, Nuriaes_ES
dc.contributor.authorDonderis, Jorgees_ES
dc.contributor.authorCiges-Tomas, J. Rafaeles_ES
dc.contributor.authorAlite, Christianes_ES
dc.contributor.authorBowring, Janine Z.es_ES
dc.contributor.authorHumphrey, Suzannees_ES
dc.contributor.authorPenadés, José R.es_ES
dc.contributor.authorMarina, Albertoes_ES
dc.date.accessioned2016-11-25T12:45:27Z-
dc.date.available2016-11-25T12:45:27Z-
dc.date.issued2016-06-20-
dc.identifier.citationNucleic Acids Research 44(11):5457-69 (2016)es_ES
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10261/140787-
dc.description13 páginas, 5 figuras, 3 tablas, material suplementario en NAR onlinees_ES
dc.description.abstractWe have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.es_ES
dc.description.sponsorshipMinisterio de Economia y Competitividad (Spain) [BIO2013-42619-P to A.M.]; Medical Research Council (UK) [MR/M003876/1]; ERC-ADG-2014 Dut-signal (from EU) [Proposal no 670932 to J.R.P]; CSIC JAE-Doc Postdoctoral contract (Programa «Junta para la Ampliación de Estudios»), European Social Fund (to E.M.); FPU13/02880 (to J.R.C.), FPI BES-2014-068617 Predoctoral Fellowships (to C.A.). Diamond Light Source block allocation group (BAG) Proposal [MX10121]; Spanish Synchrotron Radiation Facility ALBA Proposal [2014060897]; European Community's Seventh Framework Programme [FP7/2007-2013]; BioStruct-X [283570]. Funding for open access charge: Ministerio de Economia y Competitividad (Spain) [BIO2013-42619-P to A.M.]; Medical Research Council (UK) [MR/M003876/1]; ERC-ADG-2014 Dut-signal (from EU) [Proposal no 670932 to J.R.P].es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relationMINECO/ICTI2013-2016/BIO2013-42619-Pes_ES
dc.relationMINECO/ICTI2013-2016/BES-2014-068617es_ES
dc.relationMINECO/ICTI2013-2016/BIO2013-42619-Pes_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/670932-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/283570-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/670932-
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleAnother look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the partyes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1093/nar/gkw317-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1093/nar/gkw317es_ES
dc.identifier.e-issn1362-4962-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderMedical Research Council (UK)es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderEuropean Research Council-
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000265es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
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