English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/140787
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party

AuthorsMaiques, Elisa; Quiles-Puchalt, Nuria; Donderis, Jorge ; Ciges-Tomas, J. Rafael; Alite, Christian; Bowring, Janine Z.; Humphrey, Suzanne; Penadés, José R. ; Marina, Alberto
Issue Date20-Jun-2016
PublisherOxford University Press
CitationNucleic Acids Research 44(11):5457-69 (2016)
AbstractWe have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.
Description13 páginas, 5 figuras, 3 tablas, material suplementario en NAR online
Publisher version (URL)http://dx.doi.org/10.1093/nar/gkw317
URIhttp://hdl.handle.net/10261/140787
DOI10.1093/nar/gkw317
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(IBV) Artículos
Files in This Item:
File Description SizeFormat 
2016 Nucl Acids Res 44-5457.pdf1,76 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.