English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/140539
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

The cell division protein FtsZ from Streptococcus pneumoniae exhibits a GTPase activity delay

AutorSalvarelli, Estefanía; Krupka, Marcin; Rivas, Germán ; Mingnorance, Jesús; Gómez-Puertas, Paulino ; Alfonso, Carlos ; Rico, Ana Isabel
Fecha de publicación1-sep-2015
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 290: 25081- 25089 (2015)
Resumen© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.. The cell division protein FtsZ assembles in vitro by a mechanism of cooperative association dependent on GTP, monovalent cations, and Mg2+. We have analyzed the GTPase activity and assembly dynamics of Streptococcus pneumoniae FtsZ (Spn-FtsZ). SpnFtsZ assembled in an apparently cooperative process, with a higher critical concentration than values reported for other FtsZ proteins. It sedimented in the presence of GTP as a high molecular mass polymer with a well defined size and tended to form double-stranded filaments in electron microscope preparations. GTPase activity depended on K+ and Mg2+ and was inhibited by Na+. GTP hydrolysis exhibited a delay that included a lag phase followed by a GTP hydrolysis activation step, until reaction reached the GTPase rate. The lag phase was not found in polymer assembly, suggesting a transition from an initial non-GTP-hydrolyzing polymer that switches to a GTP-hydrolyzing polymer, supporting models that explain FtsZ polymer cooperativity.
URIhttp://hdl.handle.net/10261/140539
DOI10.1074/jbc.M115.650077
Identificadoresdoi: 10.1074/jbc.M115.650077
issn: 1083-351X
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Gomez-Puertas P The cell division.pdf1,86 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.