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dc.contributor.authorSarropoulou, Elena-
dc.contributor.authorPoisa Beiro, Laura-
dc.contributor.authorFigueras Huerta, Antonio-
dc.contributor.authorNovoa, Beatriz-
dc.date.accessioned2009-06-26T09:14:47Z-
dc.date.available2009-06-26T09:14:47Z-
dc.date.issued2009-04-10-
dc.identifier.citationBMC Genomics 10: 157 (2009)en_US
dc.identifier.issn1471-2164-
dc.identifier.urihttp://hdl.handle.net/10261/14004-
dc.description18 pages, 9 figures, 3 tables and 1 additional file.-- Elena Sarropoulou ... et al.en_US
dc.description.abstract[Background] The European seabass (Dicentrarchus labrax), one of the most extensively cultured species in European aquaculture productions, is, along with the gilthead sea bream (Sparus aurata), a prospective model species for the Perciformes which includes several other commercially important species. Massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. Revealing transcripts involved in immune response and studying their relative expression enhances the understanding of the immune response mechanism and consequently also the creation of vaccines. The analysis of expressed sequence tags (EST) is an efficient and easy approach for gene discovery, comparative genomics and for examining gene expression in specific tissues in a qualitative and quantitative way.en_US
dc.description.abstract[Results] Here we describe the construction, analysis and comparison of a total of ten cDNA libraries, six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen, head kidney and brain). In total 9605 sequences representing 3075 (32%) unique sequences (set of sequences obtained after clustering) were obtained and analysed. Among the sequences several immune-related proteins were identified for the first time in the order of Perciformes as well as in Teleostei.en_US
dc.description.abstract[Conclusion] The present study provides new information to the Gene Index of seabass. It gives a unigene set that will make a significant contribution to functional genomic studies and to studies of differential gene expression in relation to the immune system. In addition some of the potentially interesting genes identified by in silico analysis and confirmed by real-time PCR are putative biomarkers for bacterial and viral infections in fish.en_US
dc.description.sponsorshipThis work was supported by the European Commission's 5th Framework Programme WEALTH (Contract No. 501984, Welfare and health in sustainable aquaculture [WEALTH]).en_US
dc.format.extent727901 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccessen_US
dc.subjectDicentrarchus labraxen_US
dc.subjectEuropean seabassen_US
dc.subjectSparus aurataen_US
dc.subjectSea breamen_US
dc.titleProfiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labraxen_US
dc.typeArtículoen_US
dc.identifier.doi10.1186/1471-2164-10-157-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1186/1471-2164-10-157en_US
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