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http://hdl.handle.net/10261/139888
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dc.contributor.author | Díez-Municio, M. | - |
dc.contributor.author | Herrero, Miguel | - |
dc.contributor.author | Herrero, Miguel | - |
dc.contributor.author | Herrero, Miguel | - |
dc.contributor.author | Rivas, Blanca de las | - |
dc.contributor.author | Muñoz, Rosario | - |
dc.contributor.author | Jimeno, M. Luisa | - |
dc.contributor.author | Moreno, F. Javier | - |
dc.date.accessioned | 2016-11-07T10:17:57Z | - |
dc.date.available | 2016-11-07T10:17:57Z | - |
dc.date.issued | 2016 | - |
dc.identifier | doi: 10.1007/s00253-016-7405-z | - |
dc.identifier | issn: 0175-7598 | - |
dc.identifier | e-issn: 1432-0614 | - |
dc.identifier.citation | Applied Microbiology and Biotechnology 100(14): 6251-6263 (2016) | - |
dc.identifier.uri | http://hdl.handle.net/10261/139888 | - |
dc.description.abstract | A new process based on enzymatic synthesis of a series of raffinose-derived oligosaccharides or raffinosyl-oligofructosides (RFOS) with degree of polymerization (DP) from 4 to 8 was developed in the presence of raffinose. This process involves a transfructosylation reaction catalyzed by an inulosucrase from Lactobacillus gasseri DSM 20604 (IS). The main synthesized RFOS were structurally characterized by nuclear magnetic resonance (NMR). According to the elucidated structures, RFOS consist of β-2,1-linked fructose unit(s) to raffinose: α-d-galactopyranosyl-(1 → 6)-α-d-glucopyranosyl-(1↔2)-β-d-fructofuranosyl-((1 ← 2)-β-d-fructofuranoside)n (where n refers to the number of transferred fructose moieties). The maximum yield of RFOS was 33.4 % (in weight respect to the initial amount of raffinose) and was obtained at the time interval of 8–24 h of transfructosylation reaction initiated with 50 % (w/v) of raffinose. Results revealed the high acceptor and donor affinity of IS towards raffinose, being fairly comparable with that of sucrose for the production of fructooligosaccharides (FOS), including when both carbohydrates coexisted (sucrose/raffinose mixture, 250 g L each). The production of RFOS was also attempted in the presence of sucrose/melibiose mixtures; in this case, the predominant acceptor-product formed was raffinose followed by a minor production of a series of oligosaccharides with varying DP. The easiness of RFOS synthesis and the structural similarities with both raffinose and fructan series of oligosaccharides warrant the further study of the potential bioactive properties of these unexplored oligosaccharides. | - |
dc.description.sponsorship | M. Díez-Municio is supported by CSIC through JAE-Pre Programme co-funded by European Social Fund (ESF). M. Herrero thanks MICINN for his Ramón y Cajal contract. The authors gratefully acknowledge Dr. A. Gonçalves for the cloning of IS. | - |
dc.publisher | Springer Nature | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.subject | Transfructosylation reaction | - |
dc.subject | Bioactive oligosaccharides | - |
dc.subject | Inulosucrase | - |
dc.subject | α-Galactosides | - |
dc.subject | Raffinose | - |
dc.title | Synthesis and structural characterization of raffinosyl-oligofructosides upon transfructosylation by Lactobacillus gasseri DSM 20604 inulosucrase | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1007/s00253-016-7405-z | - |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s00253-016-7405-z | - |
dc.date.updated | 2016-11-07T10:17:57Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
Aparece en las colecciones: | (CIAL) Artículos (CENQUIOR) Artículos (ICTAN) Artículos |
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gasseriDSM20604.pdf | 428,77 kB | Adobe PDF | Visualizar/Abrir |
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