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Título

Sunflower HaGPAT9-1 is the predominant GPAT during seed development

AutorPayá-Milans, Miriam ; Aznar-Moreno, José A. ; Balbuena, Tiago S.; Haslam, Richard P.; Gidda, Satinder K.; Pérez-Hormaeche, J.; Mullen, Robert T.; Thelen, Jay J.; Napier, Johnathan A.; Salas, Joaquín J. ; Garcés Mancheño, Rafael ; Martínez-Force, Enrique ; Venegas-Calerón, Mónica
Palabras claveEndoplasmic reticulum
Glycerol-3-phosphate acyltransferase
Helianthus annuus
Mass spectrometry
Triacylglycerol
Yeast
Fecha de publicaciónnov-2016
EditorElsevier
CitaciónPlant Science 252: 42–52 (2016)
ResumenIn oil crops, triacylglycerol biosynthesis is an important metabolic pathway in which glycerol-3-phosphate acyltransferase (GPAT) performs the first acylation step. Mass spectrometry analysis of developing sunflower (Helianthus annuus) seed membrane fractions identified an abundant GPAT, HaGPAT9 isoform 1, with a N-terminal peptide that possessed two phosphorylated residues with possible regulatory function. HaGPAT9-1 belongs to a broad eukaryotic GPAT family, similar to mammalian GPAT3, and it represents one of the two sunflower GPAT9 isoforms, sharing 90% identity with HaGPAT9-2. Both sunflower genes are expressed during seed development and in vegetative tissues, with HaGPAT9-1 transcripts accumulating at relatively higher levels than those for HaGPAT9-2. Green fluorescent protein tagging of HaGPAT9-1 confirmed its subcellular accumulation in the endoplasmic reticulum. Despite their overall sequence similarities, the two sunflower isoforms displayed significant differences in their enzymatic activities. For instance, HaGPAT9-1 possesses in vivo GPAT activity that rescues the lethal phenotype of the cmy228 yeast strain, while in vitro assays revealed a preference of HaGPAT9-1 for palmitoyl-, oleoyl- and linoleoyl-CoAs of one order of magnitude, with the highest increase in yield for oleoyl- and linoleoyl-CoAs. By contrast, no enzymatic activity could be detected for HaGPAT9-2, even though its over-expression modified the TAG profile of yeast.
Descripción62 Páginas; 7 Figuras; 3 Tablas
Versión del editorhttp://dx.doi.org/10.1016/j.plantsci.2016.07.002
URIhttp://hdl.handle.net/10261/139381
DOI10.1016/j.plantsci.2016.07.002
ISSN0168-9452
E-ISSN1873-2259
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