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iTRAQ-based proteome profiling of Saccharomyces cerevisiae and cryotolerant species Saccharomyces uvarum and Saccharomyces kudriavzevii during low-temperature wine fermentation

AutorGarcía Ríos, Estéfani ; Querol, Amparo ; Guillamón, José Manuel
Palabras claveSaccharomyces
Low temperature
Quantitative proteomic
Translation
Fecha de publicación22-jun-2016
EditorElsevier
CitaciónJournal of Proteomics 146: 70-79 (2016)
ResumenTemperature is one of the most important parameters to affect the duration and rate of alcoholic fermentation and final wine quality. Some species of the Saccharomyces genus have shown better adaptation at low temperature than Saccharomyces cerevisiae, which was the case of cryotolerant yeasts Saccharomyces uvarum and Saccharomyces kudriavzevii. In an attempt to detect inter-specific metabolic differences, we characterized the proteomic landscape of these cryotolerant species grown at 12 °C and 28 °C, which we compared with the proteome of S. cerevisiae (poorly adapted at low temperature). Our results showed that the main differences among the proteomic profiling of the three Saccharomyces strains grown at 12 °C and 28 °C lay in translation, glycolysis and amino acid metabolism. Our data corroborate previous transcriptomic results, which suggest that S. kudriavzevii is better adapted to grow at low temperature as a result of enhanced more efficient translation. Fitter amino acid biosynthetic pathways can also be mechanisms that better explain biomass yield in cryotolerant strains. Yet even at low temperature, S. cerevisiae is the most fermentative competitive species. A higher concentration of glycolytic and alcoholic fermentation enzymes in the S. cerevisiae strain might explain such greater fermentation activity.
Versión del editorhttp://dx.doi.org/10.1016/j.jprot.2016.06.023
URIhttp://hdl.handle.net/10261/138127
DOI10.1016/j.jprot.2016.06.023
ISSN1874-3919
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