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Título

Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator

AutorDurante-Rodríguez, Gonzalo ; Mancheño, Jose M. ; Díaz, Eduardo ; Carmona, Manuel
Palabras claveAzoarcus
Protein engineering
Synthetic biology
Transcriptional regulation
Fecha de publicaciónago-2016
EditorJohn Wiley & Sons
CitaciónMicrobiologyOpen 5(4): 575–581 (2016)
ResumenIn this work, we explore the refactoring of the circuitry of λ phage by engineering a new-to-nature regulator that responds to an ad hoc input signal that behaves orthogonal with respect to the host cell. We tailored a chimeric regulator, termed Qλ, between the CI protein of the λ phage and the BzdR repressor from Azoarcus sp. strain CIB that responds to benzoyl-CoA. When the Qλ was expressed in the appropriate Escherichia coli cells, it was able to reprogram the lytic/lysogenic λ phage decision according to the intracellular production of benzoyl-CoA.Our results are also an example of how generating new artificial regulators that respond to effectors of choice may be useful to control different cellular processes.
Descripción7 p.-5 fig.-1 tab.
Versión del editorhttp://dx.doi.org/ 10.1002/mbo3.352
URIhttp://hdl.handle.net/10261/138052
DOI10.1002/mbo3.352
E-ISSN2045-8827
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