English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/137780
Título

A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage

AutorFernández-Orgiler, Abel; Martínez-Jiménez, María I.; Alonso, Ana ; Alcolea, Pedro J. ; Requena, Jose M.; Thomas, María del Carmen; Blanco, Luis; Larraga, Vicente
Fecha de publicación29-abr-2016
EditorOxford University Press
CitaciónNucleic Acids Research
ResumenLeishmania infantum is a protozoan parasite that is phagocytized by human macrophages. The host macrophages kill the parasite by generating oxidative compounds that induce DNA damage. We have identified, purified and biochemically characterized a DNA polymerase θ from L. infantum (LiPolθ), demonstrating that it is a DNA-dependent DNA polymerase involved in translesion synthesis of 8oxoG, abasic sites and thymine glycol lesions. Stably transfected L. infantum parasites expressing LiPolθ were significantly more resistant to oxidative and interstrand cross-linking agents, e.g. hydrogen peroxide, cisplatin and mitomycin C. Moreover, LiPolθ-overexpressing parasites showed an increased infectivity toward its natural macrophage host. Therefore, we propose that LiPolθ is a translesion synthesis polymerase involved in parasite DNA damage tolerance, to confer resistance against macrophage aggression.
Versión del editorhttp://nar.oxfordjournals.org/content/early/2016/04/29/nar.gkw346.full.pdf+html
https://www.ncbi.nlm.nih.gov/pubmed/?term=Fern%C3%A1ndez-Orgiler%20A%5BAuthor%5D&cauthor=true&cauthor_uid=27131366
URIhttp://hdl.handle.net/10261/137780
DOI10.1093/nar/gkw346
ISSN0305-1048
E-ISSN1362-4962
Aparece en las colecciones: (IPBLN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Nucl. Acids Res.-2016-Fernández-Orgiler-4855-70.pdf13,79 MBAdobe PDFVista previa
Visualizar/Abrir
nar-00220-v-2016-File011.pdfSUPPLEMENTARY DATA2,71 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.