English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/137266
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Concentrated solutions of single-chain nanoparticles: A simple model for intrinsically disordered proteins under crowding conditions

AuthorsMoreno Segurado, Ángel J. CSIC ORCID ; Lo Verso, Federica; Arbe, Arantxa CSIC ORCID; Pomposo, José A. CSIC ORCID; Colmenero de León, Juan CSIC ORCID
Issue Date2016
PublisherAmerican Chemical Society
CitationJournal of Physical Chemistry Letters 7(5): 838-844 (2016)
AbstractBy means of large-scale computer simulations and small-angle neutron scattering (SANS), we investigate solutions of single-chain nanoparticles (SCNPs), covering the whole concentration range from infinite dilution to melt density. The analysis of the conformational properties of the SCNPs reveals that these synthetic nano-objects share basic ingredients with intrinsically disordered proteins (IDPs), as topological polydispersity, generally sparse conformations, and locally compact domains. We investigate the role of the architecture of the SCNPs in their collapse behavior under macromolecular crowding. Unlike in the case of linear macromolecules, which experience the usual transition from self-avoiding to Gaussian random-walk conformations, crowding leads to collapsed conformations of SCNPs resembling those of crumpled globules. This behavior is already found at volume fractions (about 30%) that are characteristic of crowding in cellular environments. The simulation results are confirmed by the SANS experiments. Our results for SCNPs - a model system free of specific interactions - propose a general scenario for the effect of steric crowding on IDPs: collapse from sparse conformations at high dilution to crumpled globular conformations in cell environments.
Publisher version (URL)http://dx.doi.org/10.1021/acs.jpclett.6b00144
URIhttp://hdl.handle.net/10261/137266
DOIhttp://dx.doi.org/10.1021/acs.jpclett.6b00144
Identifiersdoi: 10.1021/acs.jpclett.6b00144
e-issn: 1948-7185
Appears in Collections:(CFM) Artículos
Files in This Item:
File Description SizeFormat 
Crowding Conditions.pdf436,12 kBAdobe PDFThumbnail
View/Open
SUPPORTING INFORMATION.pdf230,93 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.