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A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes

AuthorsFernández-Moya, Sandra M.; Carrington, Mark; Estévez, Antonio M.
Issue Date15-Apr-2014
PublisherOxford University Press
CitationNucleic Acids Research
AbstractWe have compared the transcriptomes of cultured procyclic Trypanosoma brucei cells in early and late logarithmic phases and found that ∼200 mRNAs were differentially regulated. In late log phase cells, the most upregulated mRNA encoded the nucleobase transporter NT8. The 3′ untranslated region (UTR) of NT8 contains a short stem–loop cis-element that is necessary for the regulation of NT8 expression in response to external purine levels. When placed in the 3′-UTR of an unregulated transcript, the cis-element is sufficient to confer regulation in response to purines. To our knowledge, this is the first example of a discrete RNA element that can autonomously regulate gene expression in trypanosomes in response to an external factor and reveals an unprecedented purine-dependent signaling pathway that controls gene expression in eukaryotes.
Publisher version (URL)http://nar.oxfordjournals.org/content/early/2014/05/09/nar.gku358.full
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