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Toward an ICPMS-linked DNA assay based on gold nanoparticles immunoconnected through peptide sequences

AuthorsMerkoçi, Arben ; Aldavert, Marta; Tarrasón, Gema; Eritja Casadellà, Ramón; Alegret, Salvador
KeywordsGold nanoparticles
Inductively coupled plasma mass spectrometry (ICPMS)
Atomic emission spectrometry
Issue Date1-Oct-2005
PublisherAmerican Chemical Society
CitationAnalytical Chemistry - Columbus 77(19): 6500-6503 (2005)
AbstractGold nanoparticles modified with anti-mouse IgG have been used to trace oligonucleotides carrying a c-myc peptide. Two strategies, a dot-blot format as well as inductively coupled plasma mass spectrometry (ICPMS) have been used to detect the nanoparticle tracer. For both cases, oligonucleotide-peptide conjugates were first applied to a nitrocellulose membrane using a manifold attached to a suction device. After immobilization of the oligonucleotide by UV radiation, the samples were incubated with an anti-c-myc monoclonal antibody. In the case of the dot-blot format strategy, it was followed by incubation with a secondary antibody conjugated to horseradish peroxidase and development with luminol as chemiluminescent substrate. In the case of ICPMS strategy, it was followed by incubation with the secondary antibody (anti-mouse IgG) conjugated to gold nanoparticles and their ICPMS detection after dissolving. The nonspecific adsorptions were found to be around zero. The limit of detection for peptide-modified DNA was 0,2 pmol. The method may have significant potential as an important ICPMS-based nonradioactive DNA detection method for the simultaneous determination of various sequences by labeling different kinds of inorganic nanoparticles. © 2005 American Chemical Society.
Publisher version (URL)http://dx.doi.org/10.1021/ac050539l
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