English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/136527
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

AuthorsMorganti, T. ; Yahel, G.; Ribes, Marta ; Coma, Rafael
KeywordsStoring procedures
Seawater analysis
Environmental Sciences
Issue 114
Nutrition
Metabolism
Ingestion-excretion
Retention rate
In situ measurements
Benthic filter feeders
Particulate and dissolved compounds
Methodology
Filtering devices
Issue Date2016
PublisherJournal of Visualized Experiments
CitationJournal of Visualized Experiments 114 : e54221 (2016)
AbstractBenthic suspension feeders play essential roles in the functioning of marine ecosystems. By filtering large volumes of water, removing plankton and detritus, and excreting particulate and dissolved compounds, they serve as important agents for benthic-pelagic coupling. Accurately measuring the compounds removed and excreted by suspension feeders (such as sponges, ascidians, polychaetes, bivalves) is crucial for the study of their physiology, metabolism, and feeding ecology, and is fundamental to determine the ecological relevance of the nutrient fluxes mediated by these organisms. However, the assessment of the rate by which suspension feeders process particulate and dissolved compounds in nature is restricted by the limitations of the currently available methodologies. Our goal was to develop a simple, reliable, and non-intrusive method that would allow clean and controlled water sampling from a specific point, such as the excurrent aperture of benthic suspension feeders, in situ. Our method allows simultaneous sampling of inhaled and exhaled water of the studied organism by using minute tubes installed on a custom-built manipulator device and carefully positioned inside the exhalant orifice of the sampled organism. Piercing a septum on the collecting vessel with a syringe needle attached to the distal end of each tube allows the external pressure to slowly force the sampled water into the vessel through the sampling tube. The slow and controlled sampling rate allows integrating the inherent patchiness in the water while ensuring contamination free sampling. We provide recommendations for the most suitable filtering devices, collection vessel, and storing procedures for the analyses of different particulate and dissolved compounds. The VacuSIP system offers a reliable method for the quantification of undisturbed suspension feeder metabolism in natural conditions that is cheap and easy to learn and apply to assess the physiology and functional role of filter feeders in different ecosystems.
Publisher version (URL)http://dx.doi.org/10.3791/54221
URIhttp://hdl.handle.net/10261/136527
DOI10.3791/54221
ISSN1940-087X
Appears in Collections:(CEAB) Artículos
(ICM) Artículos
Files in This Item:
File Description SizeFormat 
Morganti et al. 2016_ JoVE.pdf5,62 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.