English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/13632
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Marker-free image registration of electron tomography tilt-series

AuthorsSánchez Sorzano, Carlos Óscar; Messaoudi, Cédric; Eibauer, Matthias; Bilbao-Castro, José Román; Hegerl, R.; Nickell, S.; Marco, Sergio; Carazo, José M.
Issue Date27-Apr-2009
PublisherBioMed Central
CitationBMC Bioinformatics 10:124 (2009)
Abstract[Background] Tilt series are commonly used in electron tomography as a means of collecting three-dimensional information from two-dimensional projections. A common problem encountered is the projection alignment prior to 3D reconstruction. Current alignment techniques usually employ gold particles or image derived markers to correctly align the images. When these markers are not present, correlation between adjacent views is used to align them. However, sequential pairwise correlation is prone to bias and the resulting alignment is not always optimal.
[Results] In this paper we introduce an algorithm to find regions of the tilt series which can be tracked within a subseries of the tilt series. These regions act as landmarks allowing the determination of the alignment parameters. We show our results with synthetic data as well as experimental cryo electron tomography.
[Conclusion] Our algorithm is able to correctly align a single-tilt tomographic series without the help of fiducial markers thanks to the detection of thousands of small image patches that can be tracked over a short number of images in the series.
Description11 pages, 4 figures.-- PMID: 19397789 [PubMed].-- PMCID: PMC2694187.
Publisher version (URL)http://dx.doi.org/10.1186/1471-2105-10-124
URIhttp://hdl.handle.net/10261/13632
DOI10.1186/1471-2105-10-124
ISSN1471-2105
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
Marker-free_image_registration.pdf716,48 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.