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dc.contributor.authorBabkina, Olga V.es_ES
dc.contributor.authorChutko, Catherine A.es_ES
dc.contributor.authorShashkov, Alexander A.es_ES
dc.contributor.authorDzhidzhoev, Murat S.es_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorGromova, Elizaveta S.es_ES
dc.date.accessioned2016-09-02T08:39:45Z-
dc.date.available2016-09-02T08:39:45Z-
dc.date.issued2002-09-01-
dc.identifier.citationPhotochemical and Photobiological Sciences 1 (9): 636-640 (2002)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/136256-
dc.description.abstractWe used a XeCl excimer laser with 50 ns pulses, a frequency of 0.3 Hz and a wavelength of 308 mn in appropriate conditions for the photocrosslinking of EcoRII restriction endonuclease to a 14-mer DNA duplex, containing a 5-iodo-2′-deoxyuridine residue (IdU). IdU replaced the thymidine residue within the EcoRII recognition sequence 5′-CCT/AGG. The binding of EcoRII endonuclease to the IdU-containing DNA duplex was analyzed by gel retardation assay in the presence of Ca2+ or Mg2+ ions. Photocrosslinking of EcoRII to the IdU-containing DNA duplex occurred in a pre-reactive complex formed in the presence of Ca2+ ions. Photocrosslinking yields as a function of time and UV-laser light intensity were studied.es_ES
dc.description.sponsorshipWe thank Professor Alexander Yu. Borisov for valuable discussions and Robin Rycroft for assistance in preparation of the manuscript. We are grateful to Professor Ashok Bhagwat for providing us with the pR224 plasmid containing the ecoRII gene. This study was supported by the Russian Foundation of Fundamental Investigation (project no. 01–04–48637).es_ES
dc.publisherRoyal Society of Chemistry (UK)es_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectadeninees_ES
dc.subjectdouble stranded DNAes_ES
dc.subjectBase Sequencees_ES
dc.subjectCatalysises_ES
dc.subjectOligodeoxyribonucleotideses_ES
dc.titleIodouracil-mediated photocrosslinking of DNA to EcoRII restriction endonuclease in catalytic conditionses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1039/b202028a-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1039/b202028aes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
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