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Sampling the diversity of single oceanic protists for genomic analysis

AutorSieracki, Michael E.; Massana, Ramon ; Logares, Ramiro ; Stepanauskas, R.; Vargas, Colomban de ; Poulton, N.J.
Fecha de publicación25-feb-2015
EditorAssociation for the Sciences of Limnology and Oceanography
Citación2015 Aquatic Sciences Meeting. Program Book: 102 (2015)
ResumenThe diversity of marine protists is remarkably high, especially for the smaller cells. The ecological, evolutionary, and biogeochemical function of this diversity is currently the focus of much research. One approach to elucidating this diversity and its functions is the use of single cell genomics. We sampled 8 stations from the Tara Oceans expedition in the Mediterranean Sea and Indian Ocean to demonstrate this approach. Using flow cytometry, single eukaryotic cells from communities of small plastidic and aplastidic types were sorted, lysed, and their genomes amplified. Resulting Single Amplified Genomes (SAGs) were identified using eukaryote primers, yielding 989 SAGs with good quality 18S sequences, for an overall yield of 10.8%. Underrepresented groups included dinoflagellates and ciliates, probably because these cells are typically larger than the sort region used, and the parasitic MALV lineages, likely overrepresented in standard molecular surveys. SAGs from plastidic cells were predominantly from known phototrophs and those from aplastidic cells were predominantly from less characterized heterotrophic lineages. Overall, our SAG collection represented all major groups of marine eukaryotes and included many examples of poorly described groups
DescripciónAquatic Sciences Meeting, Aquatic Sciences: Global And Regional Perspectives - North Meets South, 22-27 February 2015, Granada, Spain
Versión del editorhttp://www.sgmeet.com/aslo/granada2015/program.asp
URIhttp://hdl.handle.net/10261/136220
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