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Título

Diversity of marine microeukaryotes in the global deep ocean

AutorGiner, Caterina R.; Logares, Ramiro ; Perera-Bel, Júlia; Massana, Ramon
Fecha de publicación24-feb-2015
EditorAssociation for the Sciences of Limnology and Oceanography
Citación2015 Aquatic Sciences Meeting. Program Book: 86 (2015)
ResumenThe aim of this work was to study the diversity of bathypelagic microeukaryotes. Seawater samples were taken at around 4000 m depth in 27 stations distributed in the main oceans. Pyrosequencing was used to obtain about half a million tags from the 18S rDNA V4 region, then clustered into 2482 OTUs at 97% similarity. The relative pyrotag abundance of the 20 most abundant OTUs matched well with the results of a parallel metagenomic analysis of 18S rDNA genes, suggesting little PCR bias in the tag-approach. There was a weak trend of genetic similarity among geographically close stations and among samples from the same water-mass, but we did not find a typical global community. Instead, there were four phylogenetic groups (Collodaria, Chrysophytes, Basidiomycota and MALV-II) mixed in different proportions. The amount of phylogenetic novelty was concentrated in three hotspots accounting for 6% of pyrotags globally. Rarefaction curves suggested that there were OTUs still waiting to be discovered. Our study is an essential step for a more detailed investigation of the deep ocean microbiota and suggests idiosyncratic microeukaryotic assemblages in distinct regions
DescripciónAquatic Sciences Meeting, Aquatic Sciences: Global And Regional Perspectives - North Meets South, 22-27 February 2015, Granada, Spain
Versión del editorhttp://www.sgmeet.com/aslo/granada2015/program.asp
URIhttp://hdl.handle.net/10261/136219
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