English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/136058
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Low Temperature and Short-Term High-CO2 Treatment in Postharvest Storage of Table Grapes at Two Maturity Stages: Effects on Transcriptome Profiling

AutorRosales, Raquel ; Romero, Irene ; Fernández-Caballero, Carlos ; Escribano, M. Isabel ; Merodio, Carmen ; Sánchez Ballesta, M. Teresa
Palabras claveVitis vinifera
Microarrayanalysis
Carbondioxide
Low temperature
Postharvest
Fecha de publicación13-jul-2016
EditorFrontiers Media
CitaciónFrontiers in Plant Science 7: 1020 (2016)
ResumenTable grapes (Vitis vinifera cv. Cardinal) are highly perishable and their quality deteriorates during postharvest storage at low temperature mainly because of sensitivity to fungal decay and senescence of rachis. The application of a 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2) at 0°C reduced total decay and retained fruit quality in early and late-harvested table grapes during postharvest storage. In order to study the transcriptional responsiveness of table grapes to low temperature and high CO2 levels in the first stage of storage and how the maturity stage affect these changes, we have performed a comparative large-scale transcriptional analysis using the custom-made GrapeGen GeneChip®. In the first stage of storage, low temperature led to a significantly intense change in grape skin transcriptome irrespective of fruit maturity, although there were different changes within each stage. In the case of CO2 treated samples, in comparison to fruit at time zero, only slight differences were observed. Functional enrichment analysis revealed that major modifications in the transcriptome profile of early- and late-harvested grapes stored at 0°C are linked to biotic and abiotic stress-responsive terms. However, in both cases there is a specific reprogramming of the transcriptome during the first stage of storage at 0°C in order to withstand the cold stress. Thus, genes involved in gluconeogenesis, photosynthesis, mRNA translation and lipid transport were up-regulated in the case of early-harvested grapes, and genes related to protein folding stability and intracellular membrane trafficking in late-harvested grapes. The beneficial effect of high CO2 treatment maintaining table grape quality seems to be an active process requiring the induction of several transcription factors and kinases in early-harvested grapes, and the activation of processes associated to the maintenance of energy in late-harvested grapes.
Versión del editorhttp://dx.doi.org/10.3389/fpls.2016.01020
URIhttp://hdl.handle.net/10261/136058
DOI10.3389/fpls.2016.01020
ISSN1664-462X
Aparece en las colecciones: (ICTAN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
low_temperature_Rosales.pdf1,07 MBAdobe PDFVista previa
Visualizar/Abrir
table 1.xlsxSuplemental data34,51 kBMicrosoft Excel XMLVisualizar/Abrir
table 2.xlsxSuplemental data39,68 kBMicrosoft Excel XMLVisualizar/Abrir
table 3.xlsxSuplemental data27 kBMicrosoft Excel XMLVisualizar/Abrir
table 4.xlsxSuplemental data28,24 kBMicrosoft Excel XMLVisualizar/Abrir
table 5.xlsxSuplemental data18,95 MBMicrosoft Excel XMLVisualizar/Abrir
table 6.xlsxSuplemental data25,38 kBMicrosoft Excel XMLVisualizar/Abrir
image 1.pdfSuplemental data215,7 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.