Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/135790
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The cellular origin and malignant transformation of Waldenström macroglobulinemia |
Autor: | Paiva, Bruno; Corchete, Luis A.; Vidriales, Maria Belén; García-Sanz, Ramón; Pérez, José J.; Aires-Mejia, I.; Sánchez, Maria Luz; Bárcena, Paloma; Jiménez, Cristina; Sarasquete, María Eugenia; Mateos, Maria Victoria; Ocio, Enrique M. CSIC ORCID ; Puig, Noemi; García de Coca, Alfonso; Orfao, Alberto CSIC ORCID ; Gutiérrez, Norma Carmen; San Miguel, Jesús F. CSIC ORCID | Fecha de publicación: | 2015 | Editor: | American Society of Hematology | Citación: | Blood 125(15): 2370-2380 (2015) | Resumen: | Although information about the molecular pathogenesis of Waldenström macroglobulinemia (WM) has significantly advanced, the precise cell of origin and the mechanisms behind WM transformation from immunoglobulin-M (IgM) monoclonal gammopathy of undetermined significance (MGUS) remain undetermined. Here, we undertook an integrative phenotypic, molecular, and genomic approach to study clonal B cells from newly diagnosed patients with IgM MGUS (n = 22), smoldering (n = 16), and symptomatic WM (n = 11). Through principal component analysis of multidimensional flow cytometry data, we demonstrated highly overlapping phenotypic profiles for clonal B cells from IgM MGUS, smoldering, and symptomatic WM patients. Similarly, virtually no genes were significantly deregulated between fluorescence-activated cell sorter-sorted clonal B cells from the 3 disease groups. Interestingly, the transcriptome of the Waldenström B-cell clone was highly different than that of normal CD25<sup>-</sup>CD22<sup>+</sup> B cells, whereas significantly less genes were differentially expressed and specific WM pathways normalized once the transcriptome of the Waldenström B-cell clone was compared with its normal phenotypic (CD25<sup>+</sup>CD22<sup>+low</sup>) B-cell counterpart. The frequency of specific copy number abnormalities [+4, del(6q23.3-6q25.3), +12, and +18q11-18q23] progressively increased from IgM MGUS and smoldering WM vs symptomatic WM (18% vs 20% and 73%, respectively; P = .008), suggesting a multistep transformation of clonal B cells that, albeit benign (ie, IgM MGUS and smoldering WM), already harbor the phenotypic and molecular signatures of the malignant Waldenström clone. | URI: | http://hdl.handle.net/10261/135790 | DOI: | 10.1182/blood-2014-09-602565 | Identificadores: | doi: 10.1182/blood-2014-09-602565 e-issn: 1528-0020 issn: 0006-4971 |
Aparece en las colecciones: | (IBMCC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
71
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
59
checked on 23-feb-2024
Page view(s)
266
checked on 19-abr-2024
Download(s)
103
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.