English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/135699
Título

Increased riboflavin production by manipulation of inosine 5′-monophosphate dehydrogenase in Ashbya gossypii

AutorBuey, Ruben M.; Ledesma Amaro, Rodrigo; Balsera, Mónica ; Pereda, José M. de; Revuelta Doval, José Luis
Palabras claveAshbya gossypii
Riboflavin
Inosine 5′-monophosphate dehydrogenase
Metabolic engineering
Fecha de publicación2015
EditorSpringer
CitaciónApplied Microbiology and Biotechnology 99(22): 9577-9589 (2015)
ResumenGuanine nucleotides are the precursors of essential biomolecules including nucleic acids and vitamins such as riboflavin. The enzyme inosine-5′-monophosphate dehydrogenase (IMPDH) catalyzes the ratelimiting step in the guanine nucleotide de novo biosynthetic pathway and plays a key role in controlling the cellular nucleotide pools. Thus, IMPDH is an important metabolic bottleneck in the guanine nucleotide synthesis, susceptible of manipulation by means of metabolic engineering approaches. Herein, we report the functional and structural characterization of the IMPDH enzyme from the industrial fungus Ashbya gossypii. Our data show that the overexpression of the IMPDH gene increases the metabolic flux through the guanine pathway and ultimately enhances 40 % riboflavin production with respect to the wild type. Also, IMPDH disruption results in a 100-fold increase of inosine excretion to the culture media. Our results contribute to the developing metabolic engineering toolbox aiming at improving the production of metabolites with biotechnological interest in A. gossypii.
URIhttp://hdl.handle.net/10261/135699
DOI10.1007/s00253-015-6710-2
Identificadoresdoi: 10.1007/s00253-015-6710-2
e-issn: 1432-0614
issn: 0175-7598
Aparece en las colecciones: (IRNASA) Artículos
(IBMCC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.