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dc.contributor.authorMuñiz, Carmen-
dc.contributor.authorTeodosio, Cristina-
dc.contributor.authorMayado, Andrea-
dc.contributor.authorAmaral, Ana Teresa-
dc.contributor.authorMatarraz, Sergio-
dc.contributor.authorBárcena, Paloma-
dc.contributor.authorSánchez, Maria Luz-
dc.contributor.authorÁlvarez-Twose, Iván-
dc.contributor.authorDíez-Campelo, María-
dc.contributor.authorGarcía-Montero, Andrés-
dc.contributor.authorBlanco, Juan F.-
dc.contributor.authorCañizo, María Consuelo del-
dc.contributor.authorPino Montes, Javier del-
dc.contributor.authorOrfao, Alberto-
dc.identifierdoi: 10.1186/s13287-015-0152-8-
dc.identifiere-issn: 1757-6512-
dc.identifier.citationStem Cell Research and Therapy 6: 169 (2015)-
dc.descriptionThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License.-
dc.description.abstract[Introduction]: Mesenchymal stem cells (MSCs) are multipotent cells capable of self-renewal and multilineage differentiation. Their multipotential capacity and immunomodulatory properties have led to an increasing interest in their biological properties and therapeutic applications. Currently, the definition of MSCs relies on a combination of phenotypic, morphological and functional characteristics which are typically evaluated upon in vitro expansion, a process that may ultimately lead to modulation of the immunophenotypic, functional and/or genetic features of these cells. Therefore, at present there is great interest in providing markers and phenotypes for direct in vivo and ex vivo identification and isolation of MSCs. [Methods]: Multiparameter flow cytometry immunophenotypic studies were performed on 65 bone marrow (BM) samples for characterization of CD13high CD105+ CD45– cells. Isolation and expansion of these cells was performed in a subset of samples in parallel to the expansion of MSCs from mononuclear cells following currently established procedures. The protein expression profile of these cells was further assessed on (paired) primary and in vitro expanded BM MSCs, and their adipogenic, chondrogenic and osteogenic differentiation potential was also determined. [Results]: Our results show that the CD13high CD105+ CD45− immunophenotype defines a minor subset of cells that are systematically present ex vivo in normal/reactive BM (n = 65) and that display immunophenotypic features, plastic adherence ability, and osteogenic, adipogenic and chondrogenic differentiation capacities fully compatible with those of MSCs. In addition, we also show that in vitro expansion of these cells modulates their immunophenotypic characteristics, including changes in the expression of markers currently used for the definition of MSCs, such as CD105, CD146 and HLA-DR. [Conclusions]: BM MSCs can be identified ex vivo in normal/reactive BM, based on a robust CD13high CD105+ and CD45− immunophenotypic profile. Furthermore, in vitro expansion of these cells is associated with significant changes in the immunophenotypic profile of MSCs.-
dc.description.sponsorshipThis work was supported by grants from the Instituto de Salud Carlos III, FEDER, Ministry of Economy and Competitivity, Madrid, Spain (grant PI11/02399; RETICEF RD12/0043/0021; RTICC RD12/0036/0048); Fundación Ramon Areces, Madrid, Spain (grant CIVP16A1806); Fundación Científica de la Asociación Española Contra el Cáncer (AECC); Consejería de Sanidad, Gerencia Regional de Salud de Castilla y León (SACYL) (grant BIO/SA24/13) and Fundación Samuel Solórzano Barruso (University of Salamanca, Spain); CT was supported by a grant co-financed by the European Social Fund and the Junta de Castilla y León (Spain).-
dc.publisherBioMed Central-
dc.relation.isversionofPublisher's version-
dc.titleEx vivo identification and characterization of a population of CD13high CD105+ CD45− mesenchymal stem cells in human bone marrow-
dc.description.versionPeer Reviewed-
dc.contributor.funderEuropean Commission-
dc.contributor.funderFundación Memoria de D. Samuel Solorzano Barruso-
dc.contributor.funderJunta de Castilla y León-
dc.contributor.funderFundación Ramón Areces-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderFundación Científica Asociación Española Contra el Cáncer-
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