English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/13559
Título

The role of GAP1 gene in the nitrogen metabolism of Saccharomyces cerevisiae during wine fermentation

AutorChiva, Rosana ; Baiges, Isabel; Mas, Albert ; Guillamón, José Manuel
Palabras claveGene deletion
Functional analysis
Yeast
Ammonium
Amino acids
Permeases
Wine
Fecha de publicación16-mar-2009
EditorBlackwell Publishing
Society for Applied Microbiology
CitaciónJournal of Applied Microbiology 107(1): 235-244 (2009)
Resumen[Aim] The aim of this study was to analyze the relevance of the general amino acid permease gene (GAP1) of the wine yeast Saccharomyces cerevisiae on nitrogen metabolism and fermentation performance.
[Methods and Results] We constructed a gap1 mutant in a wine strain. We compared fermentation rate, biomass production and nitrogen consumption between the gap1 mutant and its parental strain during fermentations with different nitrogen concentrations. The fermentation capacity of the gap1 mutant strain was impaired in the nitrogen-limited and nitrogen-excessive conditions. The nitrogen consumption rate between the wild strain and the mutant was different for some amino acids, especially those affected by the Nitrogen Catabolite Repression (NCR). The deletion of GAP1 gene also modified the gene expression of other permeases.
[Conclusions] The Gap1 permease seems to be important during wine fermentations with low and high nitrogen content, not only because of its amino acid transporter role but also because of its function as an amino acid sensor.
[Significance and Impact of Study] A possible biotechnological advantage of a gap1 mutant is its scarce consumption of arginine, whose metabolism has been related to the production of the carcinogenic ethyl carbamate.
Descripción10 pages, 3 figures, 4 tables.-- PMID: 19302302 [PubMed].-- Printed version published July 2009.
Versión del editorhttp://dx.doi.org/10.1111/j.1365-2672.2009.04201.x
URIhttp://hdl.handle.net/10261/13559
DOI10.1111/j.1365-2672.2009.04201.x
ISSN1364-5072
Aparece en las colecciones: (IATA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
The role of GAP1 gene in the nitrogen metabolism.pdf231,98 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.