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Title: | Development of two loquat [Eriobotrya japonica (Thunb.) Lindl.] linkage maps based on AFLPs and SSR markers from different Rosaceae species |
Authors: | Gisbert, Ana D.; Martínez-Calvo, José; Llácer, G.; Badenes, María L.; Romero, Carlos ![]() |
Keywords: | Eriobotrya japonica (Thunb.) Lindl. SSR Transferability Colinearity Linkage maps |
Issue Date: | Apr-2009 |
Publisher: | Springer |
Citation: | Molecular Breeding 23(3): 523–538 (2009) |
Abstract: | Loquat [Eriobotrya japonica (Thunb.) Lindl.] is a Rosaceae fruit species of growing interest as an alternative to the main fruit crops. However, only a few genetic studies have been carried out on this species. This paper reports the construction of the first genetic maps of two loquat cultivars based on AFLP and microsatellite markers from Malus, Eriobotrya, Pyrus and Prunus genera. An F1 population consisting of 81 individuals, derived from the cross between ‘Algerie’ and ‘Zaozhong-6’ cultivars, was used to construct both maps. A total of 111 scorable simple sequence repeat (SSR) loci resulted from the testing of 440 SSR primer pairs in the analyzed progeny and the SSR transferability to Eriobotrya was found to be 74% from apple, 58% from pear and 49% from Prunus spp. In addition, 183 AFLP polymorphic bands were produced using 42 primer combinations. The ‘Algerie’ map was organized in 17 linkage groups covering a distance of 900 cM and comprising 177 loci (83 SSRs and 94 AFLPs) with an average marker distance of 5.1 cM. Self-incompatibility trait was mapped at the distal part of the LG17 linkage group, as previously reported in Malus and Pyrus. The ‘Zaozhong-6’ map covered 870 cM comprising 146 loci (64 SSRs and 82 AFLPs) with an average marker distance of 5.9 cM. The 44 SSRs and the 48 AFLPs share in common by both maps were essentially collinear and, moreover, the order of the 75% of apple and pear SSRs mapped in Eriobotrya was shown to be consistent across the Maloideae subfamily. As a whole, these maps represent a useful tool to facilitate loquat breeding and an interesting framework for map comparison in the Rosaceae. |
Publisher version (URL): | http://dx.doi.org/ 10.1007/s11032-008-9253-8 |
URI: | http://hdl.handle.net/10261/135355 |
DOI: | 10.1007/s11032-008-9253-8 |
ISSN: | 1380-3743 |
E-ISSN: | 1572-9788 |
Appears in Collections: | (IBMCP) Artículos |
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Carlos_Romero_MB_s11032-008-9253-8.pdf | 376,69 kB | Adobe PDF | ![]() View/Open |
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