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Self-Compatibility of Two Apricot Selections Is Associated with Two Pollen-Part Mutations of Different Nature

AuthorsVilanova, Santiago; Badenes, María L.; Burgos Ortiz, Lorenzo ; Martínez-Calvo, José; Llácer, G.; Romero, Carlos
Issue DateOct-2006
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 142(2): 629-641 (2006)
AbstractLoss of pollen-S function in Prunus self-compatible mutants has recently been associated with deletions or insertions in S-haplotype-specific F-box (SFB) genes. We have studied two self-compatible cultivars of apricot (Prunus armeniaca), Currot (SCSC) and Canino (S2SC), sharing the naturally occurring self-compatible (SC)-haplotype. Sequence analysis showed that whereas the SC-RNase is unaltered, a 358-bp insertion is found in the SFBC gene, resulting in the expression of a truncated protein. The alteration of this gene is associated with self-incompatibility (SI) breakdown, supporting previous evidence that points to SFB being the pollen-S gene of the Prunus SI S-locus. On the other hand, PCR analysis of progenies derived from Canino showed that pollen grains carrying the S2-haplotype were also able to overcome the incompatibility barrier. However, alterations in the SFB2 gene or evidence of pollen-S duplications were not detected. A new class of F-box genes encoding a previously uncharacterized protein with high sequence similarity (approximately 62%) to Prunus SFB proteins was identified in this work, but the available data rules them out of producing S-heteroallelic pollen and thus the cause of the pollen-part mutation. These results suggest that cv Canino has an additional mutation, not linked to the S-locus, which causes a loss of pollen-S activity when present in pollen. As a whole, these findings support the proposal that the S-locus products besides other S-locus independent factors are required for gametophytic SI in Prunus.
DescriptionTítulo del postprint: Self-compatibility of two Prunus armeniaca selections is associated with two pollen part mutations of different nature
Publisher version (URL)http:/​/​dx.​doi.​org/​10.​1104/​pp.​106.​083865
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