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AFLP Analysis Confirms Exclusive Maternal Genomic Contribution of Meiogynogenetic Sea Bass (Dicentrarchus labrax L.)

AuthorsFelip, Alicia ; Martínez-Rodríguez, Gonzalo ; Piferrer, Francesc ; Carrillo, Manuel ; Zanuy, Silvia
Genetic markers
Paternity testing
Sea bass
Dicentrarchus labrax
Issue DateMay-2000
CitationMarine Biotechnology 2(3): 301-306 (2000)
AbstractMeiogynogenesis was induced in the European sea bass Dicentrarchus labrax L. by fertilizing eggs with UV-irradiated sperm followed by inhibition of the second meiotic division by a cold shock. Putative gynogenetic progeny derived from three groups of breeders were analyzed for maternal inheritance using amplified fragment length polymorphism (AFLP) markers. Discrimination of fingerprints was based on male-specific bands, which were absent in females. Four of 64 MseI/EcoRI primer pairs used to analyze parental polymerase chain reaction products were selected to screen progeny for paternal AFLP markers in each group. Four to 11 diagnostic bands per fish confirmed the gynogenetic origin of the progeny. AFLP analysis determined that 89.5%, 100%, and 100% of the sea bass from groups 1, 2, and 3, respectively, were gynogenetic. Our results show that AFLP analysis is suitable for verification of gynogenesis in fish
Description6 pages, 1 figure, 1 table
Publisher version (URL)https://dx.doi.org/10.1007/s101260000000
Identifiersdoi: 10.1007/s101260000000
issn: 1436-2228
e-issn: 1436-2236
Appears in Collections:(ICM) Artículos
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