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dc.contributor.authorFillat, Ursulaes_ES
dc.contributor.authorMartín-Sampedro, Raqueles_ES
dc.contributor.authorMacaya-Sanz, Davides_ES
dc.contributor.authorMartín, J. A.es_ES
dc.contributor.authorIbarra, Davides_ES
dc.contributor.authorMartínez, María Jesúses_ES
dc.contributor.authorEugenio, Mª. E.es_ES
dc.date.accessioned2016-06-27T11:15:26Z-
dc.date.available2016-06-27T11:15:26Z-
dc.date.issued2016-02-19-
dc.identifier.citationProcess Biochemistry 51: 589–598 (2016)es_ES
dc.identifier.issn1359-5113-
dc.identifier.urihttp://hdl.handle.net/10261/134099-
dc.description36 p.-5 fig.-1 tab.es_ES
dc.description.abstractNew lignin-degrading microorganisms and their enzymes can contribute to more efficient and environmentally sound use of lignocellulosic biomass in future biorefineries. In view ofthis, 127 endophytic fungi were isolated from eucalyptus trees. Strains were tested for their ability to produce ligninolytic enzymes in agar-plate medium containing ABTS, and 21 showed positive ABTS-oxidation. Positive strains in liquid medium confirmed that five produced laccase but no peroxidase activity. These strains were identified as Hormonema sp. and Pringsheimia smilacis, of the family Dothioraceae, Ulocladium sp. (Pleosporaceae) and Neofusicoccum luteum and Neofusicoccum australe (Botryosphaeriaceae). Laccase production by fungi of the family Dothioraceae is described here for the first time.To increase laccase production from these fungi, copper sulphate and ethanol were assayed as inducers. An increase in laccase activity of 85% was found for Hormonema sp. and N. luteum and 95% for N. australe. No increase was obtained for P. smilacis. A maximum temperature of 40 ◦C is recommended for possible biotechnological applications with these four strains at pH 2 for N. luteum and N. australe, pH 4 for Hormonema sp. and pH 4–5 for P. smilacis.es_ES
dc.description.sponsorshipThe authors wish to thank the Spanish Ministry of Economy and Competitiveness (MINECO) for funding this study via Projects CTQ 2011-28503-C02-01, CTQ2011-28503-C02-02 and CTQ2013- 47158-R and Programme PTA 2011-4857-I.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/CTQ2013-47158-R-
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectEndophytic fungies_ES
dc.subjectEucalyptus woodes_ES
dc.subjectLaccaseses_ES
dc.subjectLigninolytic enzymeses_ES
dc.subjectScreeninges_ES
dc.titleScreening of eucalyptus wood endophytes for laccase activityes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.procbio.2016.02.006-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/ 10.1016/j.procbio.2016.02.006es_ES
dc.identifier.e-issn1873-3298-
dc.embargo.terms2017-02-19es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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