Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/133962
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | SETD7 regulates the differentiation of human embryonic stem cells |
Autor: | Castaño, Julio; Morera, Cristina; Sesé, Borja; Boué, Stéphanie; Bonet-Costa, Carles CSIC ; Martí, Mercè; Roque, Alicia; Jordan, Albert CSIC ORCID ; Barrero, María José | Fecha de publicación: | 18-feb-2016 | Editor: | Public Library of Science | Citación: | PLoS ONE 11(2): e0149502 (2016) | Resumen: | The successful use of specialized cells in regenerative medicine requires an optimization in the differentiation protocols that are currently used. Understanding the molecular events that take place during the differentiation of human pluripotent cells is essential for the improvement of these protocols and the generation of high quality differentiated cells. In an effort to understand the molecular mechanisms that govern differentiation we identify the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. Knock-down of SETD7 causes differentiation defects in human embryonic stem cell including delay in both the silencing of pluripotency-related genes and the induction of differentiation genes. We show that SETD7 methylates linker histone H1 in vitro causing conformational changes in H1. These effects correlate with a decrease in the recruitment of H1 to the pluripotency genes OCT4 and NANOG during differentiation in the SETD7 knockdown that might affect the proper silencing of these genes during differentiation. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0149502 | URI: | http://hdl.handle.net/10261/133962 | DOI: | 10.1371/journal.pone.0149502 | Identificadores: | doi: 10.1371/journal.pone.0149502 issn: 1932-6203 |
Aparece en las colecciones: | (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Castano-PLoS-ONE-2016-v11-e0149502.PDF | 3,27 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
14
checked on 14-feb-2024
SCOPUSTM
Citations
19
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
15
checked on 22-feb-2024
Page view(s)
331
checked on 23-abr-2024
Download(s)
253
checked on 23-abr-2024