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Inntags: small self-structured epitopes for innocuous protein tagging

AuthorsGeorgieva, Maya V.; Yahya, Galal; Ortiz, Raúl; Gallego, Carme ; Aldea, Marti
KeywordsComputational models, software, statistical methods, transcriptomics
Issue Date29-Sep-2015
PublisherNature Publishing Group
CitationNature Methods 12(10): 955-958 (2015)
AbstractProtein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques. © 2015 Nature America, Inc.
DescriptionMaya V. Georgieva et al.
Publisher version (URL)http://dx.doi.org/10.1038/NMETH.3556
Identifiersdoi: 10.1038/NMETH.3556
issn: 1548-7105
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