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Título

LC-MS based metabolomics and chemometrics study of the toxic effects of copper on Saccharomyces cerevisiae

AutorFarrés, Mireia; Piña, Benjamí; Tauler, Romà
Palabras claveCopper
DNA
Fecha de publicación31-may-2016
EditorRoyal Society of Chemistry (Great Britain)
CitaciónMetallomics
ResumenCopper containing fungicides are used to protect vineyards from fungal infections. Higher residues of copper on grapes at toxic concentrations are a potentially toxic and affect the microorganisms living in vineyards, such as Saccharomyces cerevisiae. In this study, the response of metabolic profiles of S.cerevisiae at different concentrations of copper sulphate (control, 1 mM, 3 mM and 6 mM) were analysed by liquid chromatography coupled to mass spectrometry (LC-MS) and multivariate curve resolution-alternating least squares (MCR-ALS) using an untargeted metabolomics approach. Peak areas of the MCR-ALS resolved elution profiles in control and in Cu(II)-treated samples were compared using partial least squares regression (PLSR) and PLS-discriminant analysis (PLS-DA), and the intracellular metabolites best contributing to samples discrimination were selected and identified. Fourteen metabolites showed significant concentration changes upon Cu(II) exposure, following a dose-response effect. The observed changes were consistent with the expected effects of Cu(II) toxicity, including oxidative stress and DNA damage. This research confirmed that LC-MS based metabolomics coupled to chemometric methods are a powerful approach for discerning metabolomics changes in S.cerevisiae and for elucidating modes of toxicity of environmental stressors, including heavy metals like Cu(II).
Versión del editorhttp://dx.doi.org/10.1039/C6MT00021E
URIhttp://hdl.handle.net/10261/133744
DOI10.1039/C6MT00021E
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