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http://hdl.handle.net/10261/133740
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dc.contributor.author | Condezo-Hoyos, Luis | - |
dc.contributor.author | Pérez-López, Elena | - |
dc.contributor.author | Rupérez Antón, Pilar | - |
dc.date.accessioned | 2016-06-20T08:25:07Z | - |
dc.date.available | 2016-06-20T08:25:07Z | - |
dc.date.issued | 2015 | - |
dc.identifier | doi: 10.1016/j.foodchem.2015.02.039 | - |
dc.identifier | issn: 1873-7072 | - |
dc.identifier.citation | Food Chemistry 180: 265- 271 (2015) | - |
dc.identifier.uri | http://hdl.handle.net/10261/133740 | - |
dc.description.abstract | © 2015 Elsevier Ltd. All rights reserved. Optimization and validation of evaporative light scattering detector (ELSD), aided by response surface methodology (RSM), has been developed for the liquid chromatography analysis of a wide molecular weight (MW) range of carbohydrates, including polysaccharides and oligosaccharides. Optimal experimental parameters for the ELSD detection were: 88.8 °C evaporator temperature, 77.9 °C nebulizer temperature and 1.1 standard litres per minute nitrogen flow rate. Optimal ELSD detection, used together with high performance size exclusion chromatography (HPSEC) of carbohydrates, gave a linear range from 250 to 1000 mg L-1 (R2 > 0.998), with limits of detection and quantitation of 4.83-11.67 and 16.11-38.91 mg L-1, respectively. Relative standard deviation was lower than 1.8% for intra-day and inter-day repeatability for apple pectin, inulin, verbascose, stachyose and raffinose. Recovery ranged from 103.7% to 118.3% for fructo-oligosaccharides, α-galacto-oligosaccharides and disaccharides. Optimized and validated ELSD detection is proposed for the analysis of high- to low-MW carbohydrates with high sensitivity, precision and accuracy. | - |
dc.description.sponsorship | This research was supported by project AGL2014-52572-R from the Spanish MINECO. | - |
dc.publisher | Elsevier | - |
dc.rights | closedAccess | - |
dc.subject | Oligosaccharide | - |
dc.subject | Carbohydrates | - |
dc.subject | HPSEC | - |
dc.subject | RSM | - |
dc.subject | ELSD | - |
dc.subject | Polysaccharide | - |
dc.title | Improved evaporative light scattering detection for carbohydrate analysis | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.foodchem.2015.02.039 | - |
dc.date.updated | 2016-06-20T08:25:07Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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