English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/133647
Título

Perfluoroalkylated Substance Effects in Xenopus laevis A6 Kidney Epithelial Cells Determined by ATR-FTIR Spectroscopy and Chemometric Analysis

AutorGorrochategui, Eva; Lacorte, Sílvia; Tauler, Romà; Martin, Francis I.
Palabras claveCells
Cell destruction
Fecha de publicación16-may-2016
EditorAmerican Chemical Society
CitaciónChemical Research in Toxicology 29(5): 924-932(2016)
ResumenThe effects of four perfluoroalkylated substances (PFASs), namely, perfluorobutanesulfonate (PFBS), perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS), and perfluorononanoic acid (PFNA) were assessed in Xenopus laevis A6 kidney epithelial cells by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and chemometric analysis. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to visualize wavenumber-related alterations and ANOVA-simultaneous component analysis (ASCA) allowed data processing considering the underlying experimental design. Both analyses evidenced a higher impact of low-dose PFAS-treatments (10-9 M) on A6 cells forming monolayers, while there was a larger influence of high-dose PFAS-treatments (10-5 M) on A6 cells differentiated into dome structures. The observed dose-response PFAS-induced effects were to some extent related to their cytotoxicity: the EC50-values of most influential PFAS-treatments increased (PFOS < PFNA < PFOA ≪ PFBS), and higher-doses of these chemicals induced a larger impact. Major spectral alterations were mainly attributed to DNA/RNA, secondary protein structure, lipids, and fatty acids. Finally, PFOS and PFOA caused a decrease in A6 cell numbers compared to controls, whereas PFBS and PFNA did not significantly change cell population levels. Overall, this work highlights the ability of PFASs to alter A6 cells, whether forming monolayers or differentiated into dome structures, and the potential of PFOS and PFOA to induce cell death. © 2016 American Chemical Society.
Versión del editorhttp://dx.doi.org/10.1021/acs.chemrestox.6b00076
URIhttp://hdl.handle.net/10261/133647
DOI10.1021/acs.chemrestox.6b00076
Aparece en las colecciones: (IDAEA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
CHEMAGEB_23.pdf2,78 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.