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dc.contributor.authorŠimek, Karel-
dc.contributor.authorHornák, Karel-
dc.contributor.authorJezbera, Jan-
dc.contributor.authorMašín, Michal-
dc.contributor.authorNedoma, Jirí-
dc.contributor.authorGasol, Josep M.-
dc.contributor.authorSchauer, Michael-
dc.identifierdoi: 10.1128/AEM.71.5.2381-2390.2005-
dc.identifierissn: 0099-2240-
dc.identifiere-issn: 1098-5336-
dc.identifier.citationApplied and Environmental Microbiology 71(5): 2381-2390 (2005)-
dc.description10 pages, 6 figures, 4 tables-
dc.description.abstractWe studied the effects of nutrient availability and protistan grazing on bacterial dynamics and community composition (BCC) in different parts of the canyon-shaped Římov reservoir (Czech Republic). The effects of protistan grazing on BCC were examined using a size fractionation approach. Water from the dam area with only bacteria (<0.8 μm), bacteria and heterotrophic nanoflagellates (<5 μm), or whole water were incubated in situ inside dialysis bags. Top-down or predator manipulations (size fractionation) were also combined with bottom-up or resource manipulations, i.e., transplantation of samples to the middle and upper inflow parts of the reservoir with increased phosphorus availability. Significant genotypic shifts in BCC occurred with transplantation as indicated by denaturing gradient gel electrophoresis. Using different probes for fluorescence in situ hybridization, we found that 10 to 50% of total bacteria were members of the phylogenetically small cluster of β-proteobacteria (targeted with the probe R-BT065). These rod-shaped cells of very uniform size were vulnerable to predation but very fast growing and responded markedly to the different experimental manipulations. In all the grazer-free treatments, the members of the R-BT065 cluster showed the highest net growth rates of all studied bacterial groups. Moreover, their relative abundance was highly correlated with bacterial bulk parameters and proportions of bacteria with high nucleic acid (HNA) content. In contrast, increasing protistan bacterivory yielded lower proportions of R-BT065-positive and HNA bacteria substituted by increasing proportions of the class Actinobacteria, which profited from the enhanced protistan bacterivory. Copyright © 2005, American Society for Microbiology. All Rights Reserved.-
dc.description.sponsorshipThis study was largely supported by the Grant Agency of the Czech Republic under research grants 206/05/0007 and 206/02/0003 awarded to K.S. and partly by the project MSM 123100004 (structure and functioning of the system water-soil-plant), by projects DGICYT REN2001-2120/MAR and EVK3-CT-2002-00078 awarded to J.M.G., and by a cooperation project between ASCR and CSIC. M.S. was supported by the Austrian Science Foundation (project no. P15655)-
dc.publisherAmerican Society for Microbiology-
dc.titleInfluence of Top-Down and Bottom-Up Manipulations on the R-BT065 Subcluster of β-Proteobacteria, an Abundant Group in Bacterioplankton of a Freshwater Reservoir-
dc.description.versionPeer Reviewed-
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